Lymphatic Territories (Lymphosomes) in the Rat: An Anatomical Study for Future Lymphatic Research.
Lymphatic Territories (Lymphosomes) in the Rat: An Anatomical Study for Future Lymphatic Research.
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DOI：
10.1097/prs.0000000000003776
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发表时间：
2017-11
影响因子：
3.6
通讯作者：
Scaglioni MF
中科院分区：
文献类型：
--
作者：
Suami H;Scaglioni MF
了解实验动物的精确解剖结构对于正确设计研究项目至关重要。大鼠通常用于整形外科的科学研究，因为它们在学术机构中的可用性，中等成本和用于显微外科手术的相当大的血管。在过去关于大鼠解剖学的出版物中，淋巴映射有限且不完整。本研究的目的是全面绘制大鼠浅表淋巴系统。本研究使用了27只Sprague-Dawley大鼠。吲哚菁绿色（ICG）荧光淋巴造影用于识别淋巴管和淋巴结。在全身麻醉下，在沿着背侧和内侧中线、前爪和后爪以及尾部的多个点处皮内注射ICG。用记号笔在皮肤上描记淋巴管的路线并拍照。在图形模板上绘制每只大鼠的浅表淋巴管，并使用图形软件叠加所有模板以定义淋巴管和前哨淋巴结之间的关系。ICG荧光淋巴造影能显示大鼠体表淋巴管。确定了六组区域淋巴结，并描绘了通向这些淋巴结的淋巴通路。我们的淋巴体概念被成功地应用于大鼠，与六个淋巴细胞鉴定。我们成功地在大鼠中进行了浅表淋巴映射。我们的解剖学发现可以提供更多关于正常状态下淋巴系统的信息，并促进对外科操作所产生的病理变化的理解，为未来的研究提供参考。
Understanding the precise anatomy in experimental animals is crucial to correct design of research projects. Rats are commonly used for scientific research in plastic surgery because of their availability in academic institutions, moderate cost and sizable vessels for microsurgical procedures. In past publications about rat anatomy, lymphatic mapping has been limited and incomplete. The aim of this study was to comprehensively map the superficial lymphatic system in the rat. Twenty-seven Sprague–Dawley rats were used for this study. Indocyanine green (ICG) fluorescence lymphography was used to identify the lymphatic vessels and lymph nodes. Under general anaesthesia, ICG was injected intradermally at multiple spots along the dorsal and medial midlines, front and hind paws, and tail. The course of the lymphatic vessels was traced on the skin with marker pen and photographed. The superficial lymphatic vessels in each rat were sketched on a graphic template and all the templates were superimposed using graphic software to define the relationship between the lymphatic vessel and sentinel node. ICG fluorescence lymphography was able to demonstrate the superficial lymphatic vessels in the rat. Six groups of regional lymph node/s were identified and lymphatic pathways to those nodes delineated. Our lymphosome concept was successfully applied to the rat, with six lymphosomes identified. We succeeded in performing superficial lymphatic mapping in the rat. Our anatomical findings can provide further information about the lymphatic system in the normal state and promote understanding of pathological changes generated by surgical manipulation for future studies.