Effects of Endogenous Salicylic Acid During Calcium Deficiency-Induced Tipburn in Chinese Cabbage (Brassica rapa L. ssp. pekinensis).
Effects of Endogenous Salicylic Acid During Calcium Deficiency-Induced Tipburn in Chinese Cabbage (Brassica rapa L. ssp. pekinensis).
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内源水杨酸对大白菜缺钙引起的烧伤的影响
DOI：
10.1007/s11105-015-0949-8
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发表时间：
2016
影响因子：
2.1
通讯作者：
Wang W
中科院分区：
文献类型：
--
作者：
Su T;Yu S;Yu R;Zhang F;Yu Y;Zhang D;Zhao X;Wang W
通过在改良Hoagland培养基中添加不同浓度的外源钙（Ca ~（2+））培养感病植株，证明了Ca ~（2+）缺乏是引起白菜（Brassica rapa L.）SSP. pekinensis）。内源性植物钙浓度的影响，也研究了在一个双单倍体（DH）的人口组成的100个个体，但没有相关性被发现。然后，我们检查了12个钙转运蛋白基因的表达，在细胞内钙稳态在正常和诱导的条件下，Tipburn-sensitive和Tipburn-resistant植物。大多数这些基因的表达模式在这两种植物之间存在差异。在本研究中，水杨酸（SA）对缺钙有明显的响应，缺钙处理后，感倒病植株的SA和SA β-葡萄糖苷（SAG）总量比抗病植株高1.3倍。此外，观察到的SA水平的变化与台盼蓝染色显示的细胞死亡模式相关。因此，我们推测细胞质Ca ~（2+）波动诱导的下游信号事件，以及SA信号或其他生物学事件，参与了大白菜对尖烧的防御反应。本文的在线版本（doi：10.1007/s11105-015-0949-8）包含补充材料，可供授权用户使用。
By cultivating tipburn-susceptible plants in modified Hoagland’s medium containing of gradient exogenous calcium (Ca2+), we have shown that Ca2+ deficiency is one of the main causes of tipburn in Chinese cabbage (Brassica rapa L. ssp. pekinensis). The effect of endogenous plant Ca2+ concentrations on tipburn was also studied in a doubled haploid (DH) population consisting of 100 individuals, but no correlation was found. We then examined the expression of 12 Ca2+ transporter genes that function in cytosolic Ca2+ homeostasis in both tipburn-susceptible and tipburn-resistant plants under normal and tipburn-inducing conditions. Expression patterns for most of these genes differed between the two types of plants. Salicylic acid (SA) accumulated in response to conditions of calcium deficiency in our study, and both total SA and SA β-glucoside (SAG) in tipburn-susceptible plants was ∼3-fold higher than it was in resistant plants following Ca2+ deficiency treatment. Also, the changes observed in SA levels correlated well with cell death patterns revealed by trypan blue staining. Therefore, we speculate that the cytoplasmic Ca2+ fluctuation-induced downstream signaling events, as well as SA signaling or other biological events, are involved in the plant defense response to tipburn in Chinese cabbage. The online version of this article (doi:10.1007/s11105-015-0949-8) contains supplementary material, which is available to authorized users.