Various Types of Lipases Immobilized on Dendrimer-Functionalized Magnetic Nanocomposite and Application in Biodiesel Preparation
Various Types of Lipases Immobilized on Dendrimer-Functionalized Magnetic Nanocomposite and Application in Biodiesel Preparation
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DOI：
10.1021/acs.energyfuels.7b00036
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发表时间：
2017-04-01
期刊：
影响因子：
5.3
通讯作者：
Yan, Yunjun
中科院分区：
文献类型：
--
作者：
Fan, Yanli;Ke, Caixia;Yan, Yunjun
将洋葱伯克霍尔德菌脂肪酶（BCL）、米黑根毛霉脂肪酶（RML）和皱褶假丝酵母脂肪酶（CRL）3种亚族脂肪酶共价固定在树枝状分子功能化的磁性碳纳米管上，用于催化生物柴油的制备。优化了印迹分子、有机溶剂、水、甲醇、甲醇添加温度和时间间隔等因素对生物柴油产率的影响。结果表明，生物印迹技术能显著提高三种固定化脂肪酶的催化性能。将该酶用于催化生物柴油的生产，得到的最佳条件为：BCL在含水量为5 wt.反应温度为35 ℃，以叔丁醇为反应介质，醇油摩尔比为4：1时，RML的生物柴油产率最高可达96.4%，水含量为10 wt.当反应温度为50 ℃，正辛烷为反应介质，醇油摩尔比为5：1时，生物柴油的最大转化率可达96.2%; %，反应温度40 ℃，异辛烷为反应介质，甲醇：油的摩尔比为4：1，最佳收率达到85.1%。结果表明，甲醇加入时间间隔对固定化RML和CRL生物柴油转化率的影响比BCL更明显。此外，还对废植物油与大豆油制备生物柴油进行了比较。结果表明，固定化RML在其相应的溶剂体系中对两种原料均表现出较好的催化活性。
Three sub-group lipases of Burkholderia cepacia lipase (BCL), Rhizomucor miehei lipase (RML), and Candida rugosa lipase (CRL) were covalently immobilized on dendrimer functionalized magnetic carbon nanotube and used as catalysts to catalyze biodiesel production. The effects of imprinting molecule, organic solvent, water, methanol, temperature and time interval, of methanol addition on the yield of biodiesel were optimized. The results showed that bioimprinting could greatly enhance catalytic performances of the three immobilized lipases. The obtained lipases were then employed to catalyze biodiesel production, and the achieved optimum conditions were: for BCL, water content 5 wt. %, reaction temperature 35 degrees C, and with tbutanol as reaction medium, methanol : oil molar ratio 4:1, its highest biodiesel yield attained 96.4%; for RML, water content 10 wt. %, reaction temperature 50 degrees C, n-octane as the reaction medium, methanol : oil molar ratio of 5:1, the utmost biodiesel conversion rate was up to 96.2%; and for CRL, water content 7.5 wt. %, reaction temperature 40 degrees C, isooctane as the reaction medium, methanol:oil molar ratio of 4:1, the best yield reached 85.1%. It was borne out that the effect of time interval of methanol addition on the biodiesel conversion was more obvious for the immobilized RML and CRL than BCL. Furthermore, waste vegetable oil was also explored for biodiesel preparation vs soybean oil. It reveals that the immobilized RML exhibited best catalysis toward both feedstock in its corresponding solvent systems.