Possible association of CYP17 gene polymorphisms with the onset of rheumatoid arthritis.
Possible association of CYP17 gene polymorphisms with the onset of rheumatoid arthritis.
复制标题
CYP17 基因多态性与类风湿性关节炎的发病可能存在关联。
DOI：
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发表时间：
1999
影响因子：
3.7
通讯作者：
S. Hukuda
中科院分区：
文献类型：
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作者：
J. Huang;T. Ushiyama;K. Inoue;K. Mori;S. Hukuda
目的
性激素在类风湿性关节炎（RA）中的病因作用已被讨论。细胞色素P450 c 17 α（CYP 17）调节类固醇生成，CYP 17基因的限制性片段长度多态性（RFLP）与血清性激素的产生有关。本研究探讨了CYP 17基因RFLPs与RA的关系。
方法
从91名男性和285名女性RA患者以及380名男性和579名女性对照的外周血中提取基因组DNA，并测定CYP 17基因的RFLPs（表示为A1和A2等位基因）。记录RA患者的临床变量。
结果
CYP 17基因型分布在男性RA患者和女性RA患者与男性对照之间均无显著性差异。具有A2等位基因的RA患者倾向于在比不具有A2等位基因的患者更年轻的年龄发生疾病（男性50.1岁vs 54.7岁，p = 0.15;女性43.9岁vs 47.4岁，p = 0.038）。在女性中，具有A2等位基因是在老年时发生RA的弱保护因素（比值比：0.63，95%置信区间：0.41-0.95，p = 0.026）。
结论
CYP 17基因的RFLP可能通过性激素的产生而构成疾病修饰因子。
OBJECTIVE
The etiologic role of sex hormones in rheumatoid arthritis (RA) has been discussed. Cytochrome P450c 17 alpha (CYP17) regulates steroidogenesis and the restriction fragment length polymorphisms (RFLPs) of the CYP17 gene are related to serum sex hormone production. In this study, the relationship between CYP17 gene RFLPs and RA was investigated.
METHODS
Genomic DNA was extracted from the peripheral blood of 91 male and 285 female patients with RA, as well as from 380 male and 579 female controls, and the RFLPs of the CYP17 gene (denoted as the A1 and A2 alleles) were determined. Clinical variables were recorded for the RA patients.
RESULTS
There were no significant differences in CYP17 genotype distribution between the male RA patients and male controls, nor between the female RA patients and female controls. RA patients with the A2 allele tended to develop the disease at a younger age than those without (in men 50.1 vs 54.7 yrs, p = 0.15; in women 43.9 vs 47.4 yrs, p = 0.038). In women, having the A2 allele was a weak protective factor against developing RA at an older age (odds ratio: 0.63, 95% confidence interval: 0.41-0.95, p = 0.026).
CONCLUSION
The RFLPs of the CYP17 gene may constitute a disease modifying factor through sex hormone production.