THE WATER-HOLDING CAPACITY OF THE STRATUM-CORNEUM MEASURED BY H-1-NMR
THE WATER-HOLDING CAPACITY OF THE STRATUM-CORNEUM MEASURED BY H-1-NMR
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DOI：
10.1111/1523-1747.ep12277392
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发表时间：
1989-07-01
影响因子：
6.5
通讯作者：
TEZUKA, T
中科院分区：
文献类型：
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作者：
YAMAMURA, T;TEZUKA, T
在-5℃温度范围内，用质子核磁共振(1H-核磁共振)法测定了无毛大鼠角质层结合水的含量。摄氏度到零下30度。用水、氯仿：甲醇(2：1)的混合溶剂或1%十二烷基硫酸钠(SDS)水溶液提取角质层时，结合水的含量下降。在-20℃以上，丙酮：乙醚(1：1)混合溶剂的萃取不会改变结合水的含量。丙酮提取脂类：乙醚提取法包括皮脂腺脂类、胆固醇、游离脂肪酸和神经酰胺。相比之下，氯仿-甲醇提取法提取的脂质含有更多的极性脂类，如鞘磷脂，其本身的结合水与水提取的角质层相同。这些结果表明，极性脂类溶于氯仿和甲醇的混合物可能有助于提高结合水的含量。因此，假设角质层的保水能力既取决于天然保湿因子(NMF)等吸湿性成分，也取决于角质层细胞间隙中存在的极性脂类(如鞘磷脂)。
The amount of bound water in the stratum corneum of the hairless rat was measured by using proton nuclear magnetic resonance (1H-NMR) spectrometry in the temperature range of -5.degree. C to -30.degree. C. A decrease in the bound water content was observed when the stratum corneum was extracted using water, a mixed solvent of chloroform: methanol (2:1), or a 1% sodium dodecyl sulfate (SDS) aqueous solution. However, extraction using a mixed solvent of acetone: ether (1:1) did not change the bound water content at temperatures above -20.degree. C. The lipids extracted by acetone: ether-extraction method consisted of sebaceous gland lipids, cholesterol, free fatty acids, and ceramides. In contrast, the lipids extracted by chloroform: methanol-extraction method contained more polar lipids such as sphingomyelin, which had the same amount of bound water in itself as that of the water-extracted stratum corneum. These results suggest that the polar lipids soluble in the chloroform and methanol mixture may contribute to enhance the bound water content. Therefore, it is hypothesized that the water-holding capacity of the stratum corneum is dependent on both the hygroscopic components such as the natural moisturizing factor (NMF) and the polar lipids, such as sphingolipids, existing in the intercellular spaces of the stratum corneum.