The crystal structure of DynF from the dynemicin-biosynthesis pathway of Micromonospora chersina.
The crystal structure of DynF from the dynemicin-biosynthesis pathway of Micromonospora chersina.
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DOI：
10.1107/s2053230x21012322
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发表时间：
2022-01-01
期刊：
影响因子：
--
通讯作者：
Phillips GN Jr
中科院分区：
文献类型：
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作者：
Kosgei AJ;Miller MD;Bhardwaj M;Xu W;Thorson JS;Van Lanen SG;Phillips GN Jr
测定了DYNF的晶体结构，分辨率为1.5 ä，揭示了一个二聚体的八链β桶结构，内部结合了棕榈酸。动力霉素是从樱桃小单孢菌ATCC53710中分离得到的一种天然产物。进入达尼米星的生物合成基因簇使对该簇中基因产物的体外研究能够破译它们在组装这一独特分子方面的作用。本文报道了Dynemicin生物合成基因簇中的一个基因的基因产物dyF的晶体结构。DYNIF是一个二聚体的八链β桶结构，棕榈酸结合在一个空腔中。棕榈酸的存在表明DyF可能参与了前体多烯庚烯的结合，这是烯二炔核十元环合成的中心。
The crystal structure of DynF was determined to a resolution of 1.50 Å, revealing a dimeric eight-stranded β-barrel structure with palmitic acid bound in the interior. Dynemicin is an enediyne natural product from Micromonospora chersina ATCC53710. Access to the biosynthetic gene cluster of dynemicin has enabled the in vitro study of gene products within the cluster to decipher their roles in assembling this unique molecule. This paper reports the crystal structure of DynF, the gene product of one of the genes within the biosynthetic gene cluster of dynemicin. DynF is revealed to be a dimeric eight-stranded β-barrel structure with palmitic acid bound within a cavity. The presence of palmitic acid suggests that DynF may be involved in binding the precursor polyene heptaene, which is central to the synthesis of the ten-membered ring of the enediyne core.