Sex reversal in salmonid culture: Part III. The production and performance of all-female populations of brook trout
Sex reversal in salmonid culture: Part III. The production and performance of all-female populations of brook trout
复制标题
鲑鱼养殖中的性别逆转：第三部分。
DOI：
10.1016/0044-8486(79)90015-2
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
A. Walker
中科院分区：
文献类型：
--
作者：
R. Johnstone;T. H. Simpson;A. Walker
在“游上来”后的90天内，以浓度为3 mg/kg的17α-甲基睾酮饲喂全雄性虹鳟鱼幼鱼，使其发育至性成熟。在许多标本中，尽管来自睾丸的精子是有活力的，但生殖系统的变形阻止了精子的正常表达。从成熟鱼中取出或正常表达的精子标本分别用于与未经处理的雌性混合卵子受精。这种配对的所有后代都是雌性。这表明在虹鳟鱼中，雌性是同质的。在“游上来”后的56天内，将浓度为20 mg/kg的17β-雌二醇（17β-雌二醇）添加到虹鳟鱼鱼苗中，得到的全雌性种群样本也长大成熟。从这些鱼身上取出的卵子分别与正常雄鱼表达的混合精液受精。在5对这样的配对中，有2对的后代的男女比例为2.2，这表明在虹鳟鱼中，雄性在性别上是异种交配的。结果不足以可靠地估计YY组织活力受损的程度。讨论了一种通过性别反转与正常鱼交配产生单性种群的方法。
A sample of the all-male population of rainbow trout resulting from the dietary administration of 17α-methyltestosterone at a concentration of 3 mg/kg of food to juvenile fish during the 90-day period following “swim up” was grown to sexual maturity. It was observed that in many specimens there were deformations of the reproductive system which prevented the normal expression of milt although spermatozoa from the testes were viable.Sperm specimens removed surgically or normally expressed from mature fish were separately used to fertilise the mixed ova from untreated females. All the progeny of one such pairing were females. This indicates that in the rainbow trout it is the female sex which is homogametic.Samples of the all-female population which resulted from the dietary administration of 17β-oestradiol at a concentration of 20 mg/kg of food to rainbow trout fry during the 56-day period following “swim up” were also grown to maturity. The ova stripped from these fish were separately fertilised with mixed milt expressed from normal males. The progeny of two of five such pairings had a combined male to female ratio of 2.2, indicating that in rainbow trout the male is heterogametic for sex. The results were insufficient to permit any reliable estimate of the degree of impairment of the viability of the YY constitution.A method of producing single sex populations by the mating of sex reversed with normal fish is discussed.