Adipose Tissue-Derived Stem Cells Enhance Bioprosthetic Mesh Repair of Ventral Hernias
Adipose Tissue-Derived Stem Cells Enhance Bioprosthetic Mesh Repair of Ventral Hernias
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DOI：
10.1097/prs.0b013e3181e6044f
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发表时间：
2010-09-01
影响因子：
3.6
通讯作者：
Butler, Charles E.
中科院分区：
文献类型：
--
作者：
Altman, Andrew M.;Khalek, Feras J. Abdul;Butler, Charles E.
背景资料：用于腹壁疝修补术的生物补片通过细胞浸润和血管形成与肌筋膜边缘结合。脂肪组织来源的干细胞促进组织修复和血管化，并可能增加组织整合的速率或程度。作者假设，将这些细胞引入生物假体网将导致脂肪组织来源的干细胞植入和增殖，并提高纳入的bioprostic mesh.Methods：脂肪组织来源的干细胞分离自皮下脂肪组织的同基因布朗挪威大鼠，在体外扩增，并标记有绿色荧光蛋白。另外36只大鼠接受了猪脱细胞真皮基质的嵌体腹壁疝修补术。两个12只大鼠组的细胞（1.0 × 10（6））直接注射到修复后的肌筋膜/猪脱细胞真皮基质接口或接受猪脱细胞真皮基质上的细胞已presstellation; 12只大鼠对照组不接受干细胞。结果：在2周，两个干细胞组的脂肪组织来源的干细胞移植，存活，迁移和增殖。预处理组和注射干细胞组在肌筋膜/移植物界面处向猪脱细胞真皮基质中的平均细胞浸润显著大于对照组。平均血管浸润的猪脱细胞真皮基质显着大于在两个干细胞groups than in control group.Conclusions：presstellation和注射脂肪组织来源的干细胞移植，迁移，增殖，并提高血管的猪脱细胞真皮基质移植物在肌肉筋膜/移植物界面。因此，这些细胞可以增强猪脱细胞真皮基质在腹疝修复后与腹壁的结合。（Plast。重建126：845，2010）。
Background: Bioprosthetic mesh used for ventral hernia repair becomes incorporated into the musculofascial edge by cellular infiltration and vascularization. Adipose tissue-derived stem cells promote tissue repair and vascularization and may increase the rate or degree of tissue incorporation. The authors hypothesized that introducing these cells into bioprosthetic mesh would result in adipose tissue-derived stem cell engraftment and proliferation and enhance incorporation of the bioprosthetic mesh.Methods: Adipose tissue-derived stem cells were isolated from the subcutaneous adipose tissue of syngeneic Brown Norway rats, expanded in vitro, and labeled with green fluorescent protein. Thirty-six additional rats underwent inlay ventral hernia repair with porcine acellular dermal matrix. Two 12-rat groups had the cells (1.0 x 10(6)) injected directly into the musculofascial/porcine acellular dermal matrix interface after repair or received porcine acellular dermal matrix on which the cells had been preseeded; the 12-rat control group received no stem cells.Results: At 2 weeks, adipose tissue-derived stem cells in both stem cell groups engrafted, survived, migrated, and proliferated. Mean cellular infiltration into porcine acellular dermal matrix at the musculofascial/graft interface was significantly greater in the preseeded and injected stem cell groups than in the control group. Mean vascular infiltration of the porcine acellular dermal matrix was significantly greater in both stem cell groups than in the control group.Conclusions: Preseeded and injected adipose tissue-derived stem cells engraft, migrate, proliferate, and enhance the vascularity of porcine acellular dermal matrix grafts at the musculofascial/graft interface. These cells can thus enhance incorporation of porcine acellular dermal matrix into the abdominal wall after repair of ventral hernias. (Plast. Reconstr. Surg. 126: 845, 2010.)