Slight Disruption in Intestinal Environment by Dextran Sodium Sulfate Reduces Egg Yolk Size Through Disfunction of Ovarian Follicle Growth.
Slight Disruption in Intestinal Environment by Dextran Sodium Sulfate Reduces Egg Yolk Size Through Disfunction of Ovarian Follicle Growth.
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DOI：
10.3389/fphys.2020.607369
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发表时间：
2020
影响因子：
4
通讯作者：
Yoshimura Y
中科院分区：
文献类型：
--
作者：
Nii T;Bungo T;Isobe N;Yoshimura Y
肠道环境，如微生物区系、粘膜屏障功能和细胞因子的产生影响蛋鸡的产蛋量。葡聚糖硫酸钠(DSS)是一种破坏肠道环境的制剂。此前，我们曾报道，口服葡聚糖硫酸钠(DSS：0.9g/kg bw)5天可引起蛋鸡严重的肠道炎症。然而，先前研究中的DSS浓度要高得多，以引起较轻微的肠道环境破坏，而不会出现严重症状。因此，本研究的目的是确定较低剂量的DSS对蛋鸡肠道环境和产蛋量的影响。选用330日龄白来航产蛋鸡，分别口服或不添加0.225 g/kg体重的双链淀粉28天(DSS组7只，对照组8只)。每周收集所有产卵和血浆样本。最后一次治疗后1天，收集肠道组织、肝脏、卵巢卵泡和脑垂体前叶。低浓度的DSS可引起(1)绒毛高度/隐窝深度比值、大肠封闭蛋白基因表达和盲肠微生物区系多样性的降低，(2)蛋黄重量的减少，(3)血浆中VLDLy的增加，(4)并促进卵黄前体在卵泡颗粒层基因表达模式中的积累，(5)促卵泡激素和IL-1β基因在脑垂体中的表达，以及(6)血浆脂多糖结合蛋白浓度的增加。这些结果表明，给予低浓度的DSS对肠道环境有轻微的破坏作用。这种破坏包括肠道形态较差和盲肠微生物群多样性降低。肠道环境的变化会减少蛋黄的大小，但不会减少肝脏对VLDLy的供应。卵黄大小的减少可能是由于卵泡对卵黄前体的摄取功能障碍所致。总之，口服小剂量DSS是一种对肠道环境造成轻微破坏的有效方法，肠道状况通过卵巢卵泡的功能障碍而使蛋黄变小。
Intestinal environments such as microbiota, mucosal barrier function, and cytokine production affect egg production in laying hens. Dextran sodium sulfate (DSS) is an agent that disrupts the intestinal environment. Previously, we reported that the oral administration of dextran sodium sulfate (DSS: 0.9 g/kg BW) for 5 days caused severe intestinal inflammation in laying hens. However, the DSS concentration in the previous study was much higher to induce a milder disruption of the intestinal environment without heavy symptoms. Thus, the goal of this study was to determine the effects of a lower dose of DSS on the intestinal environment and egg production in laying hens. White Leghorn laying hens (330-day old) were oral administered with or without 0.225 g DSS/kg BW for 28 days (DSS and control group: n = 7 and 8, respectively). Weekly we collected all laid eggs and blood plasma samples. Intestinal tissues, liver, ovarian follicles, and the anterior pituitary gland were collected 1 day after the final treatment. Lower concentrations of orally administered DSS caused (1) a decrease in the ratio of villus height/crypt depth, occludin gene expressions in large intestine and cecal microbiota diversity, (2) a decrease in egg yolk weight, (3) an increase in VLDLy in blood plasma, (4), and enhanced the egg yolk precursor accumulation in the gene expression pattern in the follicular granulosa layer, (5) an increase in FSH and IL-1β gene expression in the pituitary gland, and (6) an increase in concentration of plasma lipopolysaccharide binding protein. These results suggested that the administration of the lower concentration of DSS caused a slight disruption in the intestinal environment. This disruption included poor intestinal morphology and decreased cecal microbiome diversity. The change in the intestinal environment decreases egg yolk size without decreasing the VLDLy supply from the liver. The decrease in egg yolk size is likely to be caused by the dysfunction of egg-yolk precursor uptake in ovarian follicles. In conclusion, the oral administration of a lower dose of DSS is an useful method to cause slight disruptions of intestinal environment, and the intestinal condition decreases egg yolk size through disfunction of ovarian follicle.