Host proteins associated with Hepatitis C virus encoded NS4A
Host proteins associated with Hepatitis C virus encoded NS4A
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DOI：
10.1007/s13337-014-0240-x
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发表时间：
2014-12-01
期刊：
影响因子：
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通讯作者：
Kaul, Rajeev
中科院分区：
文献类型：
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作者：
Dabral, Prerna;Khera, Lohit;Kaul, Rajeev
病毒相关癌症占所有癌症的12%以上。丙型肝炎病毒（HCV）感染了全世界近3%的人口，并且已经成为肝病的主要病原体，对公共卫生产生重大影响。HCV非结构蛋白NS 4A是一种54个氨基酸的多肽，作为NS 3丝氨酸蛋白酶的必需辅因子。我们在这里报告的蛋白质组学研究，以确定与NS 4A相关的细胞蛋白。本研究的结果显示了三种宿主细胞蛋白与NS 4A的关联，包括两种新的NS 4A相互作用伴侣。我们的数据提供了证据，涉及NS 4A与以前未报道的细胞蛋白质，包括管家蛋白GAPDH和PI 3 P-5 K，这是参与细胞蛋白质运输到细胞核的复合物。这些新的关联增加了与病毒感染和随后的发病机制相关的NS 4A功能的多样性。
Virus-associated cancers account for more than 12 % of all the cancers. Hepatitis C virus (HCV) infects nearly 3 % of the population worldwide and has emerged as a major causative agent of liver disease with a big impact on public health. The HCV non-structural protein NS4A is a 54-amino-acid polypeptide that serves as an essential co-factor for the NS3 serine protease. We report here on a proteomic study to identify cellular proteins associated with NS4A. The results of this study show an association of three host cellular proteins with NS4A including two novel NS4A interacting partners. Our data provide evidence for complex involving NS4A with previously unreported cellular proteins including housekeeping protein GAPDH, and PI3P-5 K which is involved in cellular protein trafficking to nucleus. These novel associations add to the diversity of NS4A functions in relation to the virus infection and subsequent pathogenesis.