CD271-selected mesenchymal stem cells from adipose tissue enhance cartilage repair and are less angiogenic than plastic adherent mesenchymal stem cells
CD271-selected mesenchymal stem cells from adipose tissue enhance cartilage repair and are less angiogenic than plastic adherent mesenchymal stem cells
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DOI：
10.1038/s41598-019-39715-z
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发表时间：
2019-02-28
影响因子：
4.6
通讯作者：
Johnson, William E. B.
中科院分区：
文献类型：
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作者：
Kohli, Nupur;Al-Delfi, Ibtesam R. T.;Johnson, William E. B.
CD271 是骨髓 MSC 的标记物，具有增强的骨或软骨修复分化能力。然而，来自脂肪组织 (AT) 的 CD271+ MSC 的性质尚不清楚。在这里，我们研究了塑料贴壁 MSC (PA MSC) 与来自 AT 的 CD271+ MSC 的分化、伤口愈合和血管生成能力。 PA MSC和CD271+ MSC在体外形成成骨细胞、脂肪细胞或软骨细胞的程度没有差异。相比之下，与 PA MSC 和对照动物相比，移植到无胸腺大鼠体内的 CD271+ MSC 显着增强了骨软骨伤口愈合，同时修复组织中的血管生成减少。在所有动物中几乎没有成熟关节软骨形成的组织学证据。 CD271+ MSC 培养物的条件培养基比 PA MSC 条件培养基的血管生成更少，并且对体外内皮细胞迁移或内皮小管形成影响很小。 CD271+ MSC 的低血管生成活性和移植时骨软骨损伤的早期组织修复得到改善，以及诱导时沿间充质谱系具有相当的分化能力，表明这些选定的细胞是比 PA MSC 更好的软骨组织修复候选细胞。
CD271 is a marker of bone marrow MSCs with enhanced differentiation capacity for bone or cartilage repair. However, the nature of CD271+ MSCs from adipose tissue (AT) is less well understood. Here, we investigated the differentiation, wound healing and angiogenic capacity of plastic adherent MSCs (PA MSCs) versus CD271+ MSCs from AT. There was no difference in the extent to which PA MSCs and CD271+ MSCs formed osteoblasts, adipocytes or chondrocytes in vitro. In contrast, CD271+ MSCs transplanted into athymic rats significantly enhanced osteochondral wound healing with reduced vascularisation in the repair tissue compared to PA MSCs and control animals; there was little histological evidence of mature articular cartilage formation in all animals. Conditioned medium from CD271+ MSC cultures was less angiogenic than PA MSC conditioned medium, and had little effect on endothelial cell migration or endothelial tubule formation in vitro. The low angiogenic activity of CD271+ MSCs and improved early stage tissue repair of osteochondral lesions when transplanted, along with a comparable differentiation capacity along mesenchymal lineages when induced, suggests that these selected cells are a better candidate than PA MSCs for the repair of cartilaginous tissue.