Factors affecting the binding of gallium-67 in serum.
Factors affecting the binding of gallium-67 in serum.
复制标题
影响血清中镓67结合的因素。
DOI：
10.1016/0047-0740(80)90107-2
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
E. Camargo
中科院分区：
文献类型：
--
作者：
M. Tsan;U. Scheffel;K. Tzen;E. Camargo
一般认为，静脉注射微量67 Ga后，放射性核素与血浆蛋白结合，主要是转铁蛋白。然而，有一些关于蛋白质载体的确切性质的讨论，以前的研究已经产生了不同的结果（1-4！HARTMAN和HAYES的一项体外研究表明，67 Ga在血清中与相对较大的分子结合。另一方面，GUNASEKERA et al.@）报道67 Ga是蛋白质结合的，约70 μ l，松散地附着于血清白蛋白、转铁蛋白和触珠蛋白。HARA“~”得出结论，放射性核素在电泳场中主要以游离化合物的形式存在，并部分与转铁蛋白结合。这似乎与CLAUSEN等人的结果形成鲜明对比，CLAUSEN等人报道，除了与a-脂蛋白的弱结合外，Ga与转铁蛋白牢固结合。本研究的目的是确定血清中67 Ga结合的性质，以及影响其结合的因素。
IT IS generally believed that after intravenous administration of trace amounts of 67Ga, the radionuclide is bound to plasma proteins, chiefly transferrin. However, there is some discussion concerning the exact nature of the protein carrier, and previous studies have yielded divergent results (1-4! An in vitro study by HARTMAN and HAYES (‘) suggested that 67Ga was bound in serum to relatively large molecules. On the other hand, GUNASEKERA et al.@) reported that 67Ga is protein-bound, about 70”/, being loosely attached to serum albumin, transferrin and haptoglobin. HARA’~’concluded that the radionuclide behaved in the electrophoretic field mostly as a free compound and was partially associated with transferrin. This appears to be in marked contrast with the results of CLAUSEN et al t4) who reported that, apart from a weak binding to be; a-lipoprotein, Ga was firmly bound to transferrin. The purpose of this study is to determine the nature of 67Ga binding in the serum, as well as the factors affecting its binding.