Candidate gene screen for potential interaction partners and regulatory targets of the Hox gene labial in the spider Parasteatoda tepidariorum
Candidate gene screen for potential interaction partners and regulatory targets of the Hox gene labial in the spider Parasteatoda tepidariorum
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DOI：
10.1007/s00427-020-00656-7
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发表时间：
2020-02-08
影响因子：
2.4
通讯作者：
Prpic，Nikola-Michael
中科院分区：
文献类型：
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作者：
Schomburg，Christoph;Turetzek，Natascha;Prpic，Nikola-Michael
Hox genelabial（实验室）控制昆虫和蜘蛛三头脑部分的形成。然而，在两组中，唇裂产生的形态学是非常不同的。在昆虫中，三脑节（蝶间节）减少，没有附属物，而在蜘蛛中，相应的节（肢肢节）是一个适当的节，包括一对附属物（肢肢）。昆虫和蜘蛛之间的这种差异很可能是由调控靶点或相互作用的伙伴介导的。然而，我们只在昆虫中发现了少数这样的基因，在蜘蛛中则没有。我们在蜘蛛中进行了候选基因筛选，使用已知（潜在）与劳动相互作用的黑腹果蝇基因在蝶间段表达。我们已经学习了75P。Tepidariorumgenes（包括先前发表的和重复的基因）。其中只有3个（proboscipedia （pb-A）和2个类似于extralenle (exd)）在腿和肢之间表现出差异表达。低成功率指出了候选基因方法在应用于谱系特异性器官时的弱点。蜘蛛的须肢在昆虫中没有对应物，因此依靠昆虫的数据显然无法识别涉及其规格和形成的大量因素。我们认为，在这些情况下，基因发现的新方法可能优于候选基因方法。
The Hox genelabial(lab) governs the formation of the tritocerebral head segment in insects and spiders. However, the morphology that results fromlabaction is very different in the two groups. In insects, the tritocerebral segment (intercalary segment) is reduced and lacks appendages, whereas in spiders the corresponding segment (pedipalpal segment) is a proper segment including a pair of appendages (pedipalps). It is likely that this difference betweenlabaction in insects and spiders is mediated by regulatory targets or interacting partners oflab. However, only a few such genes are known in insects and none in spiders. We have conducted a candidate gene screen in the spiderParasteatoda tepidariorumusing as candidatesDrosophila melanogastergenes known to (potentially) interact withlabor to be expressed in the intercalary segment. We have studied 75P. tepidariorumgenes (including previously published and duplicated genes). Only 3 of these (proboscipedia-A(pb-A) and two paralogs ofextradenticle(exd)) showed differential expression between leg and pedipalp. The low success rate points to a weakness of the candidate gene approach when it is applied to lineage specific organs. The spider pedipalp has no counterpart in insects, and therefore relying on insect data apparently cannot identify larger numbers of factors implicated in its specification and formation. We argue that in these cases a de novo approach to gene discovery might be superior to the candidate gene approach.