Palatal shelf movement during palatogenesis: a fate map of the fetal mouse palate cultured in vitro
Palatal shelf movement during palatogenesis: a fate map of the fetal mouse palate cultured in vitro
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DOI：
10.1007/s00429-004-0379-0
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发表时间：
2004-04-01
期刊：
影响因子：
--
通讯作者：
Shiota, K
中科院分区：
文献类型：
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作者：
Chou, MJ;Kosazuma, T;Shiota, K
将第13天(Plug Day=0 d)小鼠胚胎腭部在腭架的不同部位进行碳颗粒标记，并在特定的化学介质中培养48h，在培养期间，两侧的腭架接触并相互融合，模拟体内的腭裂发生。碳研究表明，在腭中区，腭架的内侧边缘上升到水平面，向中线延伸，并与相对的搁板的内侧边缘接触。另一方面，在陆架前后两端附近，陆架内侧边缘通过改建形成了一些新的组织，这些新形成的组织参与了腭裂的融合。本研究结果表明，在小鼠腭裂发生过程中，腭架的前部和后部与腭中部有不同的表现。结果表明，在胎鼠上颌骨中，上颌骨的闭合主要是通过口盖的旋转和内侧伸长，而前后部的闭合主要是通过内侧边缘的组织重塑，部分是通过口盖的内侧拉长。
Day-13 fetal mouse palates (plug day=day 0) were labeled with carbon particles at various sites of palatal shelves and cultivated in a chemically defined medium for up to 48 h. During the culture period, the bilateral palatal shelves came in contact and fused with each other, which simulated in vivo palatogenesis. The carbon study revealed that at the midpalatal region, the medial edge of the palatal shelf elevated to the horizontal plane, elongated toward the midline, and made contact with the medial edge of the opposing shelf. On the other hand, near the anterior and posterior ends of the shelf, some new tissue was formed at the medial edge of the shelf by remodeling and this newly formed tissue took part in palatal fusion. The results of the present study indicate that during mouse palatogenesis, the anterior and posterior regions of the palatal shelf behave differently from the midpalatal region. It seems that in the fetal mouse palate, the midpalate closes mainly by means of rotation and medial elongation of the shelf, whereas the anterior and posterior parts of the palate close mainly by tissue remodeling of the medial edge and partly by medial elongation of the shelf.