Iron uptake by immature erythroid cells. Mechanism of dependence on metabolic energy.
Iron uptake by immature erythroid cells. Mechanism of dependence on metabolic energy.
复制标题
未成熟红细胞对铁的吸收。
DOI：
10.1016/0005-2736(77)90013-x
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
E. Morgan
中科院分区：
文献类型：
--
作者：
S. Kailis;E. Morgan
使用兔网织红细胞和骨髓细胞研究了转铁蛋白结合铁的利用与细胞代谢相关的机制。通过使用作用于代谢途径不同位点的抑制剂（NaF、氟乙酸钠、鱼藤酮、2,4-二硝基苯酚、NaCN）和添加代谢底物（肌苷、丙酮酸钠、乳酸钠）来改变代谢速率。在许多实验中，测量了铁和转铁蛋白的吸收率，以及细胞 ATP 和 NADH 浓度。结果表明，铁摄取率与细胞内ATP浓度存在显着相关性，但与NADH浓度无相关性。转铁蛋白摄取速率受到的抑制程度小于铁摄取的抑制程度，并且仅当 ATP 浓度降至抑制铁摄取所需的浓度以下时。结论是，未成熟红系细胞摄取转铁蛋白结合铁的速率取决于细胞内ATP浓度，但与NADH浓度无关。
The mechanism by which the utilization of transferrin-bound iron is linked with cellular metabolism was investigated using rabbit reticulocytes and bone marrow cells. The rate of metabolism was altered by the use of inhibitors which act at different sites in the metabolic pathway (NaF, sodium fluoroacetate, rotenone, 2,4-dinitrophenol, NaCN) and by the addition of metabolic substrates (inosine, sodium pyruvate, sodium lactate). Measurements were made of the rates of iron and transferrin uptake and, in many of the experiments, of cellular ATP and NADH concentrations. The results showed that there was a significant correlation between the rate of iron uptake and the ATP concentration of the cells, but no correlation was found with the NADH concentration. The rate of transferrin uptake was inhibited to a lesser degree than that of iron uptake, and only when the ATP concentration had fallen below that necessary to inhibit iron uptake. It is concluded that the rate of uptake of transferrin-bound iron by immmature erythroid cells is dependent on the intracellular concentration of ATP but is independent of the NADH concentration.