Microalgae carotenoids intake: Influence on cholesterol levels, lipid peroxidation and antioxidant enzymes
Microalgae carotenoids intake: Influence on cholesterol levels, lipid peroxidation and antioxidant enzymes
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DOI：
10.1016/j.foodres.2019.108770
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发表时间：
2020-02-01
影响因子：
8.1
通讯作者：
Zepka, Leila Queiroz
中科院分区：
文献类型：
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作者：
do Nascimento, Tatiele Casagrande;Betim Cazarin, Cinthia Bau;Zepka, Leila Queiroz
本研究的目的是评估斜生栅藻摄入类胡萝卜素对脂质过氧化、内源性抗氧化防御系统以及体内血清脂质谱的影响。将雄性小鼠分为对照组，并补充不同剂量的微藻类胡萝卜素：0.25（MC1）和2.5（MC2）mg.kg(-1)体重。测定血清样本中的脂质谱（总胆固醇、甘油三酯、低密度脂蛋白和高密度脂蛋白）和肝毒性标志物。测定心脏、肝脏、肾脏和脾脏中的抗氧化酶和硫代巴比妥酸反应物质。用于治疗动物的两种剂量均未显示出肝毒性标志物的不良影响。 MC1 不会引起血清脂质谱的显着变化。相比之下，它显着减少了脾脏的脂质过氧化（46%），并增加了心脏中的 GR（40%）和肾脏中的 GPx（79%）活性。 MC2 治疗还增加了心脏中的 GR (49%) 以及心脏和肾脏 (58%) 中的 GPx (243%) 活性，但显着增加了肝脏中的脂质过氧化水平 (160%) 以及血清甘油三酯 (60%)。结果表明，食用 MC1 剂量的斜生类胡萝卜素对动物是安全的，并且可以探索作为提高抗氧化酶活性和减少脂质过氧化的替代方案。
The objective of this study was to evaluate the effect of carotenoids intake of Scenedesmus obliquus, on lipid peroxidation, the endogenous antioxidant defense system as well as the serum lipid profile in vivo. Male mice were divided into control groups and supplemented with different doses of microalgae carotenoids: 0.25 (MC1) and 2.5 (MC2) mg.kg(-1) bodyweight. The lipid profile (total cholesterol, triglycerides, low and high-density lipoprotein) and markers of hepatic toxicity were determined in serum samples. Antioxidant enzymes and thiobarbituric acid reactive substances were determined in the heart, liver, kidneys, and spleen. Both doses used to treat the animals did not show adverse effects by markers of hepatic toxicity. MC1 did not cause significant changes in the serum lipid profile. In contrast, it created a significant reduction in lipid peroxidation of the spleen (46%) as well as an increase in the GR in the heart (40%) and GPx in the kidneys (79%) activity. The MC2 treatment also increased GR (49%) in the heart and GPx (243%) in the heart and kidneys (58%) activity, however, significantly increased levels of lipid peroxidation in the liver (160%) as well as serum triglycerides (60%). According to results, it is suggested that the consumption of S. obliquus carotenoids at the MC1 dose was safe to the animals and could be explored as an alternative to improve the activity of antioxidant enzymes and reduce lipid peroxidation.