ΦC31 integrase mediates efficient cassette exchange in the zebrafish germline.
ΦC31 integrase mediates efficient cassette exchange in the zebrafish germline.
复制标题
φC31整合酶介导斑马鱼种系中的有效盒式交换。
DOI：
10.1002/dvdy.22699
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发表时间：
2011-09
影响因子：
2.5
通讯作者：
Fisher, Shannon
中科院分区：
文献类型：
--
作者：
Hu, Gui;Goll, Mary G.;Fisher, Shannon
Site specific recombinases (SSRs) are powerful tools for genome manipulation, used in diverse organisms including Drosophila melanogaster, mouse, Arabidopsis, zebrafish, and human cultured cells. The integrase from the bacteriophage ΦC31 belongs to the large serine family of integrases, and in contrast to other widely used SSRs such as Cre and Flp, recombination is directional and therefore irreversible. We have developed a vector system for recombinase mediated cassette exchange (RMCE) in the zebrafish, allowing swapping of the coding sequence in an integrated transgene. Utilizing codon–optimized ΦC31 integrase RNA bearing the 3′UTR from the nanos1 gene, we replaced the egfp coding sequence of an integrated reporter transgene with mCherry coding sequence. Recombination was achieved at high efficiency in both somatic cells and in the germline. We demonstrate an effective approach to RMCE, increasing the repertoire of tools available to manipulate the zebrafish genome.
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影响因子：
3
作者：
Lu J;Maddison LA;Chen W
通讯作者：
Chen W
影响因子：
2.7
作者：
Davison, Jon M.;Akitake, Courtney M.;Parsons, Michael J.
通讯作者：
Parsons, Michael J.
影响因子：
3.3
作者：
Bateman, Jack R.;Lee, Anne M.;Wu, C. -ting
通讯作者：
Wu, C. -ting
影响因子：
2.7
作者：
Akitake, Courtney M.;Macurak, Michelle;Goll, Mary G.
通讯作者：
Goll, Mary G.
DOI：
10.1073/pnas.97.21.11403
发表时间：
2000-10-10
影响因子：
11.1
作者：
Kawakami, K;Shima, A;Kawakami, N
通讯作者：
Kawakami, N