The time-course analysis of gene expression during wound healing in mouse skin
The time-course analysis of gene expression during wound healing in mouse skin
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DOI：
10.1016/j.legalmed.2008.09.004
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发表时间：
2009-03-01
期刊：
影响因子：
1.5
通讯作者：
Nakasono, Ichiro
中科院分区：
文献类型：
--
作者：
Kagawa, Shinichiro;Matsuo, Aya;Nakasono, Ichiro
RNA分析已应用于法医工作，以确定伤口的年龄。我们使用定量RT-PCR技术检测了包括c-fos、fosB、丝裂原活化蛋白激酶磷酸酶-1（MKP-1）、CD 14、趋化因子（C-C基序）配体9（CCL 9）、胎盘生长因子（PlGF）、肥大细胞蛋白酶-5（MCP-5）、生长停滞特异性5（Gas 5）、β-2微球蛋白（B2 M）和主要尿蛋白-1（MUP-1）在内的基因的mRNA表达。在成年小鼠的修复反应方面。c-fos、fosB和MKP-1转录本的表达水平在1h内达到高峰，CD 14和CCL 9转录本的表达水平在12 ~ 24 h达到高峰。PIGF和MCP-5 mRNA的增加出现在约第5天。Gas 5、B2 M和MUP-1转录本无明显变化。各基因随时间的变化具有差异表达模式。结果提示，对创伤皮肤中7个基因的观察有助于创伤年龄的准确诊断。(c)2009年由Elsevier爱尔兰有限公司出版。
RNA analysis has been applied to forensic work to determine wound age. We investigated mRNA expression using quantitative RT-PCR often genes, including c-fos, fosB, mitogen-activated protein kinase phosphatase-1 (MKP-1), CD14, chemokine (C-C motif) ligand 9 (CCL9), placenta growth factor (PIGF), mast cell protease-5 (MCP-5), growth arrest specific 5 (Gas5), beta-2 microglobulin (B2M) and major urinary protein-1 (MUP-1). in terms of repair response in adult mice. The expression level of c-fos, fosB and MKP-1 transcripts increased drastically, peaked within I h, and that of the CD14 and CCL9 transcripts peaked from 12 to 24 h. An increase in PIGF and MCP-5 mRNA appeared on about day 5. Gas5, B2M and MUP-1 transcripts showed no significant change. Each gene had differentially expressional patterns with time-course. Our result implied that the observation of the 7 genes in wounded skin could serve to aid in the accurate diagnosis of wound age. (c) 2009 Published by Elsevier Ireland Ltd.