CILIARY NEUROTROPHIC FACTOR PREVENTS RETROGRADE NEURONAL DEATH IN THE ADULT CENTRAL-NERVOUS-SYSTEM
CILIARY NEUROTROPHIC FACTOR PREVENTS RETROGRADE NEURONAL DEATH IN THE ADULT CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1073/pnas.90.6.2222
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发表时间：
1993-03-15
影响因子：
11.1
通讯作者：
KOLIATSOS, VE
中科院分区：
文献类型：
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作者：
CLATTERBUCK, RE;PRICE, DL;KOLIATSOS, VE
神经细胞因子睫状神经营养因子（CNTF）最初被描述为一种在体外支持鸡睫状神经节神经元存活的活性。CNTF及其主要结合蛋白CNTF受体α在中枢和外周神经系统中的广泛表达表明该肽具有更广泛的营养作用。在本研究中，我们报告了CNTF可以防止成年大鼠丘脑前腹侧和前嗅侧核中由axocut诱导的神经元细胞死亡。利用聚合酶链反应，我们也证明了CNTF和CNTF受体α mRNA在这些丘脑核中的存在。CNTF及其受体在响应该因子的神经元群体中的巧合表明CNTF具有旁分泌功能。本研究证实了CNTF在体内对中枢神经系统神经元有显著影响，并证明了神经细胞因子可以预防成人中枢神经系统细胞死亡。
The neurocytokine ciliary neurotrophic factor (CNTF) was described originally as an activity that supports the survival of neurons of the chicken ciliary ganglia in vitro. The widespread expression of CNTF and its principal binding protein, CNTF receptor alpha, in the central and peripheral nervous systems suggests a broader trophic role for this peptide. In the present study, we report that CNTF prevents axotomy-induced cell death of neurons in the anteroventral and anterodorsal thalamic nuclei of the adult rat. Using the polymerase chain reaction, we also demonstrate the presence of CNTF and CNTF receptor alpha mRNA in these same thalamic nuclei. The coincidence of CNTF and its receptor in a population of neurons responding to the factor suggests a paracrine function for CNTF. The present findings establish that CNTF has significant effects on neurons of the central nervous system in vivo and demonstrate that neurocytokines can prevent cell death in the adult central nervous system.