MALAT1 long ncRNA promotes gastric cancer metastasis by suppressing PCDH10.
MALAT1 long ncRNA promotes gastric cancer metastasis by suppressing PCDH10.
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MALAT1长ncRNA通过抑制PCDH10促进胃癌转移
DOI：
10.18632/oncotarget.7281
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发表时间：
2016-03-15
期刊：
影响因子：
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通讯作者：
Zhao X
中科院分区：
文献类型：
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作者：
Qi Y;Ooi HS;Wu J;Chen J;Zhang X;Tan S;Yu Q;Li YY;Kang Y;Li H;Xiong Z;Zhu T;Liu B;Shao Z;Zhao X
EZH 2是多梳抑制复合物2（PRC 2）的催化组分，在人类癌症中经常过表达，并部分通过肿瘤抑制基因的转录沉默促进肿瘤的发生和发展。许多非编码RNA（ncRNA）将EZH 2募集到特定的染色质位点，在那里它们调节基因表达。在这里，我们使用RNA免疫沉淀测序（RIP-seq）来分析人胃癌细胞系中EZH 2相关转录物。我们鉴定了8，256个转录本，包括非编码和编码转录本，其中一些来自癌症相关基因座。特别是，我们发现长链非编码RNA（lncRNA）MALAT 1结合EZH 2，抑制肿瘤抑制因子PCDH 10，并促进胃细胞迁移和侵袭。因此，我们的工作提供了EZH 2相关转录组的全局视图，并为EZH 2在胃肿瘤发生中的功能提供了新的见解。
EZH2, the catalytic component of polycomb repressive complex 2 (PRC2), is frequently overexpressed in human cancers and contributes to tumor initiation and progression, in part through transcriptional silencing of tumor suppressor genes. A number of noncoding RNAs (ncRNAs) recruit EZH2 to specific chromatin loci, where they modulate gene expression. Here, we used RNA immunoprecipitation sequencing (RIP-seq) to profile EZH2-associated transcripts in human gastric cancer cell lines. We identified 8,256 transcripts, including both noncoding and coding transcripts, some of which were derived from cancer-related loci. In particular, we found that long noncoding RNA (lncRNA) MALAT1 binds EZH2, suppresses the tumor suppressor PCDH10, and promotes gastric cellular migration and invasion. Our work thus provides a global view of the EZH2-associated transcriptome and offers new insight into the function of EZH2 in gastric tumorigenesis.