Evidence that murine V gamma 5 and V gamma 6 gamma delta-TCR+ lymphocytes are derived from a common distinct lineage.
Evidence that murine V gamma 5 and V gamma 6 gamma delta-TCR+ lymphocytes are derived from a common distinct lineage.
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有证据表明小鼠 V gamma 5 和 V gamma 6 gamma delta-TCR 淋巴细胞源自共同的不同谱系。
作者：
K. Heyborne;Y. Fu;H. Kalataradi;C. Reardon;C. Roark;C. Eyster;M. Vollmer;W. Born;R. O’Brien
小鼠V γ 5和V γ 6+ T淋巴细胞在胎儿和新生儿期间在胸腺中依次发育，产生表皮和雌性生殖道的上皮内淋巴细胞。在分析一组来源于各种组织的γ δ T细胞杂交瘤时，我们发现所有测试的V γ 6+细胞（n = 25）表达V γ 5-C γ 1 mRNA，而所分析的一组V γ 6-细胞（n = 33）均不表达该mRNA。V γ 6 mRNA在V γ 5+细胞中罕见（9个中的1个），并且在所有其他γ δ T细胞中不存在（n = 10）。这些发现表明，大多数（如果不是全部的话）V γ 6-TCR+淋巴细胞来自先前重排了V γ 5基因的前体细胞，并表明V γ 5和V γ 6+淋巴细胞属于共同的、不同的谱系，其不产生其他γ δ T细胞。
Murine V gamma 5 and V gamma 6+ T lymphocytes develop sequentially in the thymus during fetal and newborn life, giving rise to intraepithelial lymphocytes of the epidermis and female reproductive tract. In analyzing a panel of gamma delta T cell hybridomas derived from various tissues, we found that all V gamma 6+ cells tested (n = 25) expressed V gamma 5-C gamma 1 mRNA, whereas none of a panel of V gamma 6- cells analyzed (n = 33) expressed this mRNA. V gamma 6 mRNA was rare in V gamma 5+ cells (1 of 9), and absent in all other gamma delta T cells (n = 10). These findings suggest that most, if not all, V gamma 6-TCR+ lymphocytes arise from precursor cells that have previously rearranged the V gamma 5 gene, and indicate that V gamma 5 and V gamma 6+ lymphocytes belong to a common, distinct lineage that does not give rise to other gamma delta T cells.