Application of a circulating-cathodic-antigen (CCA) strip test and real-time PCR, in comparison with microscopy, for the detection of Schistosoma haematobium in urine samples from Ghana
Application of a circulating-cathodic-antigen (CCA) strip test and real-time PCR, in comparison with microscopy, for the detection of Schistosoma haematobium in urine samples from Ghana
复制标题
DOI：
10.1179/136485908x337490
复制
发表时间：
2008-10-01
影响因子：
--
通讯作者：
van Lieshout, L.
中科院分区：
文献类型：
--
作者：
Obeng, B. B.;Aryeetey, Y. A.;van Lieshout, L.
在寄生虫感染的检测中，与较新的分子检测相比，基于显微镜的传统方法通常具有较低的灵敏度和/或特异性。目前，在埃及血吸虫感染检测中，基于实时 PCR 的检测方法和用于检测循环阴极抗原 (CCA) 的试剂条测试均已与尿液过滤和显微镜检查进行了比较。尿液样本取自加纳埃及沙门氏菌流行地区的 74 名“病例”和非流行地区的 79 名“对照者”，分别使用这三种方法进行检查。以过滤和显微镜检查结果为“金标准”，实时 PCR 的特异性为 100%，敏感性为 89%，而 CCA 试纸条的特异性为 91%，敏感性为 41%。当发现样本中含有≥50个卵/10ml（表明感染相对较重）时，PCR和CCA的灵敏度较高，分别为100%和62%。正如预期的那样，鸡蛋计数与 PCR 中所需的扩增循环数呈负相关，以给出超出背景的信号（r=-0.38；P
In the detection of parasitic infection, the traditional methods based on microscopy often have low sensitivity and/or specificity compared with the newer, molecular tests. An assay based on real-time PCR and a reagent strip test for detecting circulating cathodic antigen (CCA) have both now been compared with urine filtration and microscopy, in the detection of Schistosoma haematobium infections. Urine samples, obtained from 74 'cases' in areas of Ghana with endemic S. haematobium and 79 'controls' fromnon-endemic areas, were each checked using the three methods. With the results of the filtration and microscopy taken as the 'gold standard', real-time PCR was found to be 100% specific and 89% sensitive whereas the CCA strips were 91% specific and 41% sensitive. With the samples found to contain >= 50 eggs/10 ml (indicating relatively intense infections), the sensitivities of the PCR and CCA were higher, at 100% and 62%, respectively. As expected, egg counts were negatively correlated with the number of amplification cycles needed, in the PCR, to give a signal that exceeded the background (r=-0.38; P