Self-Assembled Monolayers Derived from Positively Charged Adsorbates on Plasmonic Substrates for MicroRNA Delivery: A Review
Self-Assembled Monolayers Derived from Positively Charged Adsorbates on Plasmonic Substrates for MicroRNA Delivery: A Review
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DOI：
10.3390/jnt4020009
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发表时间：
2023-05
期刊：
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通讯作者：
J. Hoang;P. Tajalli;Mina Omidiyan;Maria D. Marquez;Orawan Khantamat;W. Tuntiwechapikul;Chien-Hung Li-Chien-Hung
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文献类型：
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作者：
J. Hoang;P. Tajalli;Mina Omidiyan;Maria D. Marquez;Orawan Khantamat;W. Tuntiwechapikul;Chien-Hung Li-Chien-Hung
微小RNA（miRNA）已成为癌症的一种有前途的替代治疗方法，但其递送受到循环期间细胞摄取和降解低的阻碍。在这篇综述中，我们讨论了递送miRNA的各种方法，包括病毒和非病毒递送系统，如脂质体和纳米颗粒。我们还研究了纳米颗粒在基于miRNA的诊断中的应用。我们特别关注非病毒递送系统，利用钴金属纳米颗粒的形式和使用自组装单分子层（SAM）作为表面改性的方法。我们回顾了使用自组装膜的共轭和小的非编码核糖核酸（ncRNA），特别是自组装膜来自带正电荷的吸附物产生带电的表面，可以与带负电荷的miRNA静电相互作用。我们还讨论了金和其他等离子体纳米粒子的细胞摄取的影响，以及与寡核苷酸降解相关的挑战。我们的综述强调了基于SAM的系统作为体外和体内递送miRNA和其他RNA的通用和强大工具的潜力，以及进一步研究以解决与miRNA递送和诊断相关的挑战的必要性。
MicroRNA (miRNA) has emerged as a promising alternative therapeutic treatment for cancer, but its delivery has been hindered by low cellular uptake and degradation during circulation. In this review, we discuss the various methods of delivering miRNA, including viral and non-viral delivery systems such as liposomes and nanoparticles. We also examine the use of nanoparticles for miRNA-based diagnostics. We focus specifically on non-viral delivery systems utilizing coinage metals in the form of nanoparticles and the use of self-assembled monolayers (SAMs) as a method of surface modification. We review the use of SAMs for the conjugation and delivery of small noncoding ribonucleic acid (ncRNA), particularly SAMs derived from positively charged adsorbates to generate charged surfaces that can interact electrostatically with negatively charged miRNA. We also discuss the effects of the cellular uptake of gold and other plasmonic nanoparticles, as well as the challenges associated with the degradation of oligonucleotides. Our review highlights the potential of SAM-based systems as versatile and robust tools for delivering miRNA and other RNAs in vitro and in vivo and the need for further research to address the challenges associated with miRNA delivery and diagnostics.