Unraveling secrets of telomeres: one molecule at a time.
Unraveling secrets of telomeres: one molecule at a time.
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DOI：
10.1016/j.dnarep.2014.01.012
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发表时间：
2014-08
期刊：
影响因子：
3.8
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Lin J;Kaur P;Countryman P;Opresko PL;Wang H
端粒在维持线性染色体的稳定性方面发挥着重要作用。端粒维持涉及多种蛋白质与长重复序列和复杂的动态 DNA 结构（例如 G 四链体、T 环和 T 环）相互作用的动态作用。鉴于端粒的异质性和复杂性，单分子方法对于充分理解控制端粒维持的结构-功能关系至关重要。在这篇综述中，我们简要概述了单分子成像和操作技术的原理。然后，我们重点介绍应用这些单分子技术研究 G-四链体、端粒酶和庇护蛋白的结构、动力学和功能所获得的结果。
Telomeres play important roles in maintaining the stability of linear chromosomes. Telomere maintenance involves dynamic actions of multiple proteins interacting with long repetitive sequences and complex dynamic DNA structures, such as G-quadruplexes, T-loops and t-circles. Given the heterogeneity and complexity of telomeres, single-molecule approaches are essential to fully understand the structure-function relationships that govern telomere maintenance. In this review, we present a brief overview of the principles of single-molecule imaging and manipulation techniques. We then highlight results obtained from applying these single-molecule techniques for studying structure, dynamics and functions of G-quadruplexes, telomerase, and shelterin proteins.