The immune adherence receptor CR1-like existed on porcine erythrocytes membrane.
The immune adherence receptor CR1-like existed on porcine erythrocytes membrane.
复制标题
猪红细胞膜上存在类免疫粘附受体CR1。
DOI：
10.1038/srep13290
复制
发表时间：
2015-08-13
影响因子：
4.6
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Yin W;Cui J;Jiang J;Zhao J;Fan K;Sun N;Wang Z;Sun Y;Ma H;Li H
本研究获得了一株鼠抗猪补体受体1（CR 1）样单克隆抗体（McAb），并利用该McAb证实了猪红细胞上存在CR 1样蛋白。我们的研究结果证实，CR 1样蛋白定位于猪红细胞的表面。小鼠免疫球蛋白G抑制血清调理的绿色荧光蛋白表达大肠杆菌与猪红细胞的结合。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析表明，类CR 1单抗与生化纯化的猪红细胞膜组分发生反应，在135 ~ 140 kDa处有一条清晰的带。我们推测，135 kDa至140 kDa的膜蛋白是猪红细胞CR 1样蛋白的等价物。
In the present study, we obtain a mouse anti-porcine complement receptor type 1 (CR1)-like monoclonal antibody (McAb) and use this McAb to verify the existence of CR1-like protein on porcine erythrocytes. Our results confirm that CR1-like protein is localized on the surface of porcine erythrocytes. Mouse immunoglobulin G inhibited the binding of serum-opsonized green fluorescent protein-expressing Escherichia coli to porcine erythrocytes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis indicates that CR1-like McAb reacts with biochemically-purified porcine erythrocyte membrane fractions, with a clear band at 135 kDa to 140 kDa. We postulate that the 135 kDa to 140 kDa membrane protein is the equivalent of the porcine erythrocyte CR1-like protein.