Fabrication of highly catalytic silver nanoclusters/graphene oxide nanocomposite as nanotag for sensitive electrochemical immunoassay.
Fabrication of highly catalytic silver nanoclusters/graphene oxide nanocomposite as nanotag for sensitive electrochemical immunoassay.
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DOI：
10.1016/j.aca.2015.12.018
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发表时间：
2016-02
影响因子：
6.2
通讯作者：
Jiamian Wang;Xiuyun Wang;Shuo Wu;Jie Song;Yanqiu Zhao;Yanqiu Ge;C. Meng
中科院分区：
文献类型：
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作者：
Jiamian Wang;Xiuyun Wang;Shuo Wu;Jie Song;Yanqiu Zhao;Yanqiu Ge;C. Meng
制备了银纳米簇和氧化石墨烯纳米复合物（AgNCs/GRO），并用检测抗体进行了功能化，用于高灵敏度的电化学检测癌胚抗原（CEA）， CEA是许多癌症的模型肿瘤标志物。以DNA为模板合成了具有大表面积和丰富低配位位点的agnc，对H2O2的电化学还原具有较高的催化活性。由于GRO具有较大的比表面积、超强的电子导电性以及与DNA疏水性碱基之间强的π-π堆叠相互作用，可以进一步提高agnc的催化能力，因此采用GRO与agnc进行组装。以agnc /GRO为信号扩增标签，设计了一种无酶的电化学免疫传感方案，在捕获抗体功能化免疫传感界面上对CEA进行高灵敏度检测。在最佳条件下，所设计的免疫传感器具有0.1 pg mL−1 ~ 100 ng mL−1的宽线性范围，低检测限为0.037 pg mL−1。实际样品分析表明，该传感器具有良好的准确性和重复性，表明agnc /GRO在制造高灵敏度免疫传感器方面具有很大的应用前景，可扩展到各种低丰度疾病相关蛋白的检测。
Silver nanoclusters and graphene oxide nanocomposite (AgNCs/GRO) is synthesized and functionalized with detection antibody for highly sensitive electrochemical sensing of carcinoembryonic antigen (CEA), a model tumor marker involved in many cancers. AgNCs with large surface area and abundant amount of low-coordinated sites are synthesized with DNA as template and exhibit high catalytic activity towards the electrochemical reduction of H2O2. GRO is employed to assemble with AgNCs because it has large specific surface area, super electronic conductivity and strong π-π stacking interaction with the hydrophobic bases of DNA, which can further improve the catalytic ability of the AgNCs. Using AgNCs/GRO as signal amplification tag, an enzyme-free electrochemical immunosensing protocol is designed for the highly sensitive detection of CEA on the capture antibody functionalized immunosensing interface. Under optimal conditions, the designed immunosensor exhibits a wide linear range from 0.1 pg mL−1to 100 ng mL−1and a low limit of detection of 0.037 pg mL−1. Practical sample analysis reveals the sensor has good accuracy and reproducibility, indicating the great application prospective of the AgNCs/GRO in fabricating highly sensitive immunosensors, which can be extended to the detection of various kinds of low abundance disease related proteins.