The genetic diversity of narcissus viruses related to turnip mosaic virus blur arbitrary boundaries used to discriminate potyvirus species
The genetic diversity of narcissus viruses related to turnip mosaic virus blur arbitrary boundaries used to discriminate potyvirus species
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DOI：
10.1371/journal.pone.0190511
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发表时间：
2018-01-04
期刊：
影响因子：
3.7
通讯作者：
Gibbs, Adrian J.
中科院分区：
文献类型：
--
作者：
Ohshima, Kazusato;Mitoma, Shinichiro;Gibbs, Adrian J.
水仙（Narcissus tazetta var.从日本各地收集显示花叶或条纹叶的中国大豆（chinensis），并使用马铃薯Y病毒特异性引物测试病毒感染。许多人被发现感染了一种macluravirus和不同马铃薯Y病毒的混合物，其中三分之一是水仙黄条病毒（NYSV）样病毒。对9种NYSV样病毒的基因组进行了测序，并与4种已经发表的病毒一起，提供了它们在芜菁花叶病毒（TuMV）系统发育组中的位置的系统发育和成对身份分析的数据。使用现有的ICTV标准来定义马铃薯Y病毒属物种，发现TuMV组中的水仙病毒来自五个物种;先前描述的NLSYV，以及我们称为水仙病毒1（NV-1）和水仙黄条病毒-1至3（NYSV-1，NYSV-2和NYSV-3）的四个新物种。然而，由于所有病毒都来自同一宿主种，并且在中国和印度已经发现了NV-1和NYSV-3 '亲本的天然重组体，我们也可以得出结论，它们可以被认为是一个单一的巨型种水仙病毒的成员;定义这种马铃薯Y病毒属物种的标准是它们的多蛋白序列具有大于69%的相同核苷酸和大于75%的相同核苷酸。相同的氨基酸
Narcissus plants (Narcissus tazetta var. chinensis) showing mosaic or striping leaves were collected from around Japan, and tested for virus infections using potyvirus-specific primers. Many were found to be infected with a macluravirus and mixtures of different potyviruses, one third of them narcissus yellow stripe virus (NYSV)-like viruses. Genomes of nine of the NYSV-like viruses were sequenced and, together with four already published, provided data for phylogenetic and pairwise identity analyses of their place in the turnip mosaic virus (TuMV) phylogenetic group. Using existing ICTV criteria for defining potyvirus species, the narcissus viruses in TuMV group were found to be from five species; the previously described NLSYV, and four new species we call narcissus virus 1 (NV-1) and narcissus yellow stripe-1 to -3 (NYSV-1, NYSV-2 and NYSV-3). However, as all are from a single host species, and natural recombinants with NV-1 and NYSV-3 'parents have been found in China and India, we also conclude that they could be considered to be members of a single mega-species, narcissus virus; the criteria for defining such a potyvirus species would then be that their polyprotein sequences have greater than 69% identical nucleotides and greater than 75% identical amino acids.