Mechanisms for flow-enhanced cell adhesion
Mechanisms for flow-enhanced cell adhesion
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DOI：
10.1007/s10439-008-9464-5
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发表时间：
2008-04-01
影响因子：
3.8
通讯作者：
McEver, Rodger P.
中科院分区：
文献类型：
--
作者：
Zhu, Cheng;Yago, Tadayuki;McEver, Rodger P.
细胞粘附是由特定的受体-配体键介导的。在一些生物系统中，尽管位移流体剪切力增加，但已观察到增加的流量可以增强细胞粘附。流动增强的细胞粘附包括几个方面：流动增强了流动细胞对静止表面的初始束缚，减慢了流动细胞的速度并增加了滚动细胞的规律性，增加了滚动粘附细胞的数量。这种有趣现象的机制可能包括依赖于传输的键形成加速和依赖于力的键解离减速。前者包括三种不同的传输模式：细胞底部在表面的滑动、细胞的布朗运动和相互作用分子的旋转扩散。后者涉及一种最近被证明的反直觉行为，称为捕获键，其中力延长而不是缩短受体-配体键的寿命。在本文中，我们总结了最近发表的数据，利用量纲分析和突变分析来阐明l -选择素-配体相互作用介导的流动增强白细胞粘附的上述机制。
Cell adhesion is mediated by specific receptor-ligand bonds. In several biological systems, increasing flow has been observed to enhance cell adhesion despite the increasing dislodging fluid shear forces. Flow-enhanced cell adhesion includes several aspects: flow augments the initial tethering of flowing cells to a stationary surface, slows the velocity and increases the regularity of rolling cells, and increases the number of rollingly adherent cells. Mechanisms for this intriguing phenomenon may include transport-dependent acceleration of bond formation and force-dependent deceleration of bond dissociation. The former includes three distinct transport modes: sliding of cell bottom on the surface, Brownian motion of the cell, and rotational diffusion of the interacting molecules. The latter involves a recently demonstrated counterintuitive behavior called catch bonds where force prolongs rather than shortens the lifetimes of receptor-ligand bonds. In this article, we summarize our recently published data that used dimensional analysis and mutational analysis to elucidate the above mechanisms for flow-enhanced leukocyte adhesion mediated by L-selectin-ligand interactions.