Adrenomedullin 2/intermedin-like immunoreactivity in the hypothalamus and brainstem of rats
Adrenomedullin 2/intermedin-like immunoreactivity in the hypothalamus and brainstem of rats
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DOI：
10.1016/j.autneu.2008.01.003
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发表时间：
2008-05-30
影响因子：
2.7
通讯作者：
Ueta, Yoichi
中科院分区：
文献类型：
--
作者：
Hashimoto, Hirofumi;Kitamura, Kazuo;Ueta, Yoichi
用免疫组织化学方法观察了侧脑室注射秋水仙碱（100 μ g/rat）和慢性盐负荷（2%生理盐水饮用）5天后大鼠脑内肾上腺髓质素2（AM 2）（与中间肽相同）样免疫反应性（LI）。在溶剂处理和正常水化大鼠中，在下丘脑和脑干中观察到AM 2-LI神经元，包括终板的血管器、正中视前核、视上核（SON）、室旁核（PVN）、下丘脑腹内侧核、弓状核、蓝斑、孤束核和疑核。在秋水仙碱处理和盐负荷大鼠，AM 2-LI神经元更强烈地可视化在SON和PVN的大细胞部分比在每个控制。在秋水仙素处理的大鼠PVN的小细胞部分出现了一些AM 2-LI神经元，而盐负荷大鼠则没有。在秋水仙素处理和慢性盐负荷后，下丘脑和脑干的其他区域的AM 2-LI没有改变。这些结果表明，下丘脑和脑干中的AM 2/intermedin可能在神经内分泌和自主神经功能，如水/盐平衡中发挥作用。(c)2008 Elsevier B. V.保留所有权利。
Adrenomedullin 2 (AM2) (identical to intermedin)-like immunoreactivity (LI) was examined in the rat brain by immunohistochemistry after intracerebroventricular administration of colchicine (100 mu g/rat) and chronic salt loading (2% saline to drink) for 5 days. In both vehicle-treated and euhydrated rats, AM2-LI neurons were observed in the hypothalamus and brainstem, including in the organum vasculosum of the lamina terminalis, the median preoptic nucleus, the supraoptic nucleus (SON), the paraventricular nucleus (PVN), the ventromedial hypothalamic nucleus, the arcuate nucleus, the locus coeruleus, the nucleus of the tractus solitarius and the nucleus ambiguus. In colchicine-treated and salt loaded rats, AM2-LI neurons were visualized more strongly in the SON and the magnocellular part of the PVN than in those in each control. Some AM2-LI neurons appeared in the parvocellular part of the PVN in the colchicine-treated but not salt loaded rats. AM2-LI in the other areas of the hypothalamus and brainstem did not change after colchicine-treatment and chronic salt loading. These results suggest that AM2/intermedin in the hypothalamus and brainstem may play roles on neuroendocrine and autonomic functions, such as water/salt balance, in rats. (c) 2008 Elsevier B.V. All rights reserved.