Study on morphology and lubrication of articular cartilage surface with atomic force microscopy
Study on morphology and lubrication of articular cartilage surface with atomic force microscopy
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原子力显微镜研究关节软骨表面形态及润滑
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
M. Horimoto
中科院分区：
文献类型：
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作者：
Y. Sawae;T. Murakami;Kenji Matsumoto;M. Horimoto
利用原子力显微镜（AFM）在生理溶液中检查天然关节软骨表面的形态和结构特征，并通过往复滑动试验研究软骨表面的润滑能力。将刚切下的软骨标本立即浸入磷酸盐缓冲盐水(PBS)中，并通过流体敲击法在充满PBS的AFM的液体池中进行观察。 AFM图像显示，关节软骨表面非常光滑，有1～2μm高的平缓小丘，在生理湿润条件下覆盖有凝胶层。软骨素酶 ABC 可以消化凝胶层并暴露纤维网络。因此，可以认为该表面层的主要成分是蛋白聚糖。滑动试验结果表明，关节软骨与玻璃板之间的摩擦力最初很低，随着重复滑动逐渐增大。摩擦软骨表面的AFM图像显示，低摩擦阶段保留了表面凝胶层，而高摩擦阶段出现了纤维结构。这些结果表明，表面凝胶层对恶劣操作条件下的自然滑膜关节润滑发挥着重要作用。
Morphological and structural characteristics of natural articular cartilage surface were examined in a physiological solution by using an atomic force microscopy (AFM), and the lubricating ability of a cartilage surface was investigated in a reciprocating sliding tests. Freshly excised cartilage specimens were immediately immersed in phosphate-buffered saline (PBS), and observed in the liquid cell of the AFM filled with PBS by the fluid tapping method. AFM images revealed that the surface of articular cartilage was very smooth, with gentle mounds of 1∼2 μm height, and covered with a gel layer under the physiological wet condition. Chondroitinase ABC could digest the gel layer and expose the fibrous networks. Therefore, it could be considered that the main constituent of this surface layer was proteoglycans. Results of sliding tests showed that the friction between articular cartilage and glass plate was initially very low, and increased gradually with repetition of sliding. AFM images of rubbed cartilage surface showed that the surface gel layer was preserved for the low friction stage, on the other hand, fibrous structure appeared for the higher friction stage. These results indicated that the surface gel layer play an important role on the natural synovial joint lubrication under the severe operating condition.