Methanogenic archaeal communities in rice roots grown in flooded soil pots: Estimation by PCR-DGGE and sequence analyses
Methanogenic archaeal communities in rice roots grown in flooded soil pots: Estimation by PCR-DGGE and sequence analyses
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DOI：
10.1080/00380768.2004.10408526
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发表时间：
2004-10-01
影响因子：
2
通讯作者：
Kimura, M
中科院分区：
文献类型：
--
作者：
Ikenaga, M;Asakawa, S;Kimura, M
通过16S rDNA的聚合酶链式反应-变性梯度凝胶电泳谱分析和水稻根部DNA提取液序列分析，研究了水稻根部(根际和根面)产甲烷古生菌群落。不同生长期和不同羽化节位的根样间产甲烷古菌群落的DGGE图谱基本一致，表明特定的产甲烷古菌群落成员持续存在于水稻根部。由于产甲烷古生菌的DGGE条带在水稻生长早期出苗后不久就在幼根中检测到，因此推测幼根中存在缺氧部位。根据水稻生长早期根节间DGGE格局的变化，根龄也影响了水稻根部产甲烷古生菌群落。所有测序的克隆都属于甲烷微生物群和水稻集群I，它们被认为是未培养的产甲烷古生菌成员之一。
Methanogenic archaeal communities in rice roots (endorhizosphere and rhizoplane) were studied based on the analysis of the polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) patterns of 16S rDNA and sequence analysis of DNA extracts from nodal roots of rice plants grown in flooded soil pots. DGGE patterns of the methanogenic archaeal communities were similar among the root samples, irrespective of the growth stage and emergence node, which indicated that specific methanogenic archaeal members inhabited rice roots constantly. As the DGGE bands from the methanogenic archaeal members were detected in young nodal roots soon after emergence at the early stage of rice growth, the presence of anoxic sites in young roots was estimated. Root age also affected the methanogenic archaeal communities in rice roots based on the changes in the DGGE patterns among nodal roots at the early stage of rice growth. All the sequenced clones belonged to Methanomicrobiales and Rice cluster I that had been assumed to be one of the uncultured methanogenic archaeal members.