OsBT1 encodes an ADP-glucose transporter involved in starch synthesis and compound granule formation in rice endosperm.
OsBT1 encodes an ADP-glucose transporter involved in starch synthesis and compound granule formation in rice endosperm.
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OsBT1 编码参与水稻胚乳淀粉合成和复合颗粒形成的 ADP-葡萄糖转运蛋白
DOI：
10.1038/srep40124
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发表时间：
2017-01-05
影响因子：
4.6
通讯作者：
Hu P
中科院分区：
文献类型：
--
作者：
Li S;Wei X;Ren Y;Qiu J;Jiao G;Guo X;Tang S;Wan J;Hu P
淀粉是高等植物的主要贮藏碳水化合物。尽管已经对淀粉生物合成的几种酶和调节剂进行了表征，但谷物种子中淀粉合成的完整调节网络仍然难以捉摸。在这里，我们报告的鉴定和表征的水稻Brittle 1（OsBT 1）基因，这是专门在发育胚乳中表达。osbt 1突变体的胚乳为白色，粒重显著低于野生型。osbt 1复合淀粉粒的形成和发育明显缺陷，淀粉体在发育早期即已解体，淀粉粒分散在胚乳细胞中，不能复合在胚乳细胞中。总淀粉含量和直链淀粉含量降低，淀粉的理化性质发生变化。此外，聚合度（DP）的支链淀粉在osbt 1是显着不同的野生型。图位克隆结果表明，OsBT 1编码一种嘌呤类ADP-葡萄糖转运蛋白。OsBT 1编码的蛋白定位于淀粉体被膜。此外，osbt 1突变体中淀粉合成相关基因的表达也发生了改变。这些结果表明OsBT 1在淀粉合成和复合淀粉颗粒的形成中起重要作用。
Starch is the main storage carbohydrate in higher plants. Although several enzymes and regulators for starch biosynthesis have been characterized, a complete regulatory network for starch synthesis in cereal seeds remains elusive. Here, we report the identification and characterization of the rice Brittle1 (OsBT1) gene, which is expressed specifically in the developing endosperm. The osbt1 mutant showed a white-core endosperm and a significantly lower grain weight than the wild-type. The formation and development of compound starch granules in osbt1 was obviously defective: the amyloplast was disintegrated at early developmental stages and the starch granules were disperse and not compound in the endosperm cells in the centre region of osbt1 seeds. The total starch content and amylose content was decreased and the physicochemical properties of starch were altered. Moreover, the degree of polymerization (DP) of amylopectin in osbt1 was remarkably different from that of wild-type. Map-based cloning of OsBT1 indicated that it encodes a putatively ADP-glucose transporter. OsBT1 coded protein localizes in the amyloplast envelope membrane. Furthermore, the expression of starch synthesis related genes was also altered in the osbt1 mutant. These findings indicate that OsBT1 plays an important role in starch synthesis and the formation of compound starch granules.
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影响因子：
7.2
作者：
Delatte, T;Trevisan, M;Zeeman, SC
通讯作者：
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影响因子：
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影响因子：
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通讯作者：
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