Preparation and characterization of a novel electrospun spider silk fibroin/poly(D,L-lactide) composite fiber
Preparation and characterization of a novel electrospun spider silk fibroin/poly(D,L-lactide) composite fiber
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新型静电纺蜘蛛丝蛋白/聚(D,L-丙交酯)复合纤维的制备及表征
DOI：
10.1021/jp800913k
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发表时间：
2008-09-11
影响因子：
3.3
通讯作者：
Li, Fei
中科院分区：
文献类型：
--
作者：
Zhou, Shaobing;Peng, Hongsen;Li, Fei
本文首次成功制备了蜘蛛丝素蛋白/聚乳酸复合纤维非织造毡。用扫描电镜（SEM）和透射电镜（TEM）观察了纤维的形貌。采用傅里叶变换红外光谱（FT-IR）表征了静电纺丝前后蛛丝蛋白二级结构的变化。在此，通过分析FT-IR二阶导数光谱，通过酰胺I带的去卷积获得定量信息，对丝蛋白的构象变化进行定性分析。进行机械测试以研究拉伸强度和断裂伸长率。还进行了水接触角（CA）测量以表征纤维的表面性质。基于CCL 81（Vero）细胞增殖研究评价静电纺丝PDLLA和Ss-PDLLA非织造纤维垫的细胞毒性。结果表明，蛛丝蛋白的引入提高了复合纤维的亲水性和力学性能。
In the paper, we successfully prepared spider silk fibroins (Ss)/poly(D,L-lactide) (PDLLA) composite fibrous nonwoven mats for the first time to the best of our knowledge. The morphology of the fibers was observed by a scanning electron microscope (SEM) and transmission electron microscope (TEM). The secondary structure change of the spidroin before and after electrospinning was characterized using Fourier transform infrared spectroscopy (FT-IR). Herein, a qualitative analysis of the conformational changes of the silk protein was performed by analyzing the FT-IR second-derivative spectra, from which quantitative information was obtained via the deconvolution of the amide I band. A mechanical test was carried out to investigate the tensile strength and the elongation at break. A water contact angle (CA) measurement was also performed to characterize Surface properties of the fibers. The cytotoxicity of electrospun PDLLA and Ss-PDLLA nonwoven fibrous mats was evaluated based on a CCL 81(Vero) cells proliferation study. The results showed that the hydrophilic and mechanical property of the composite fiber were improved by introducing spidroin.