Detection of antigen-specific T cells with multivalent soluble class II MHC covalent peptide complexes
Detection of antigen-specific T cells with multivalent soluble class II MHC covalent peptide complexes
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DOI：
10.1016/s1074-7613(00)80572-5
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发表时间：
1998-06-01
期刊：
影响因子：
32.4
通讯作者：
Kappler, J
中科院分区：
文献类型：
--
作者：
Crawford, F;Kozono, H;Kappler, J
多聚体可溶性 MHC II 类分子稳定地被共价连接的肽占据，以适当的特异性结合 T 细胞杂交瘤和来自 T 细胞受体转基因小鼠的 T 细胞。可溶性 T 细胞受体对单体 MHC/肽的亲和力与多聚 MHC/肽与 T 细胞的结合水平之间存在直接相关性。虽然多聚体 MHC/肽复合物的结合与 T 细胞受体亲和力和表达水平成正比，但 T 细胞 CD4 的影响很小。
Multimeric soluble MHC class II molecules stably occupied with covalently attached peptides bind with appropriate specificity to T cell hybridomas and T cells from T cell receptor transgenic mice. There is a direct correlation between soluble T cell receptor affinity for monomeric MHC/peptide and level of binding of multimeric MHC/peptide to T cells. While binding of the multimeric MHC/peptide complex is proportional to T cell receptor affinity and expression level, there is little influence of T cell CD4.