Development of a pigment-based whole-cell biosensor for the analysis of environmental copper
Development of a pigment-based whole-cell biosensor for the analysis of environmental copper
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DOI：
10.1039/c7ra03778c
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发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Yeh, Yi-Chun
中科院分区：
文献类型：
--
作者：
Chen, Pei-Hsuan;Lin, Chieh;Yeh, Yi-Chun
使用工程微生物检测环境中的重金属已被证明是非常有效和强大的。本文报道了一种新型铜离子微生物传感器的研制。为了开发这种微生物传感器，我们筛选和表征了各种生物部分，包括启动子，输出信号和宿主。此外，为了减少检测时间，我们使用植物色素甜菜黄素输出荧光信号。该全细胞生物传感器在检测淡水池塘和自来水等环境样品中的铜离子时具有良好的灵敏度。
Using engineered microorganisms to detect heavy metals in the environment has proven to be highly effective and robust. This paper reports on the development of a novel microbial sensor for the detection of copper ions. To develop this microbial sensor, we screened and characterized various biological parts, including promoters, output signals, and hosts. In addition, we used the plant pigment betaxanthin to output fluorescent signals in order to reduce the detection time. The resulting whole-cell biosensor presented a good sensitivity when detecting copper ions in environmental samples including freshwater pond and tap water.