RNA interference in neuroscience: Progress and challenges
RNA interference in neuroscience: Progress and challenges
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DOI：
10.1007/s10571-005-8447-4
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发表时间：
2005-12-01
影响因子：
4
通讯作者：
Gonzalez-Alegre, P
中科院分区：
文献类型：
--
作者：
Miller, VM;Paulson, HL;Gonzalez-Alegre, P
1. RNA干扰（RNA interference，RNAi）是近年来发现的一种介导转录后基因沉默的生物学途径。RNAi的过程是由一个越来越好理解的细胞机制精心策划的。天然和工程化RNAi的共同切入点是称为短干扰RNA（siRNA）的双链RNA分子，其介导靶向信使RNA（mRNA）的序列特异性鉴定和降解。这些siRNA的研究和操作最近已经彻底改变了生物医学研究。在这篇综述中，我们首先简要概述了RNAi的过程，重点是其在脑功能和参与神经系统疾病中的潜在作用。然后，我们描述了在实验室中操纵RNAi的方法及其在神经科学中的应用。最后，我们重点介绍了RNAi在神经系统疾病中的潜在治疗应用。
1. RNA interference (RNAi) is a recently discovered biological pathway that mediates post-transcriptional gene silencing. The process of RNAi is orchestrated by an increasingly well-understood cellular machinery.2. The common entry point for both natural and engineered RNAi are double stranded RNA molecules known as short interfering RNAs (siRNAs), that mediate the sequence-specific identification and degradation of the targeted messenger RNA (mRNA). The study and manipulation of these siRNAs has recently revolutionized biomedical research.3. In this review, we first provide a brief overview of the process of RNAi, focusing on its potential role in brain function and involvement in neurological disease. We then describe the methods developed to manipulate RNAi in the laboratory and its applications to neuroscience. Finally, we focus on the potential therapeutic application of RNAi to neurological disease.