Duration of norovirus excretion and the longitudinal course of viral load in norovirus-infected elderly patients
Duration of norovirus excretion and the longitudinal course of viral load in norovirus-infected elderly patients
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DOI：
10.1016/j.jhin.2009.12.016
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发表时间：
2010-05-01
影响因子：
6.9
通讯作者：
Matsuzaki, Y.
中科院分区：
文献类型：
--
作者：
Aoki, Y.;Suto, A.;Matsuzaki, Y.
为了防止诺如病毒在半封闭环境（如老年护理设施）中传播，了解诺如病毒感染个体的感染期非常重要。我们招募了13名年龄在60-98岁之间患有诺如病毒胃肠炎的老年患者（11名老年护理机构的居民和2名健康成年人）进行这项研究，并使用实时定量聚合酶链反应测定了63份随访粪便样本中的诺如病毒病毒载量。诺如病毒排泄的平均时间为14.3天（范围：9-32天;中位数：13天）。在症状发作后7至10天收集的所有后续样本检测结果均为阳性。在症状发作后14至18天收集的样本中的病毒载量被分为三组：检测阴性的，10（4）拷贝/g粪便的。10（4）拷贝/g粪便组的粪便显示粪便中的诺如病毒排泄延长（长达32天）。虽然排泄病毒的感染期尚未明确，但这些结果表明，应在症状发作后至少14天内密切关注，并且在发作后约16天测量粪便中的病毒载量可能是跟踪每例诺如病毒感染患者病毒脱落过程的有用方法。(C)2009年，医院感染协会。由爱思唯尔有限公司出版。保留所有权利。
To prevent dissemination of norovirus in semiclosed environments such as aged-care facilities, it is important to know the period of infectivity in norovirus-infected individuals. We recruited 13 elderly patients aged 60-98 years with norovirus gastroenteritis (11 residents in aged-care facilities and two healthy adults) for this study, and measured the viral loads for norovirus in a total of 63 follow-up faecal samples using a real-time quantitative polymerase chain reaction assay. The average period of norovirus excretion was 14.3 days (range: 9-32 days; median: 13 days). All of the follow-up samples collected between 7 and 10 days after the onset of symptoms tested positive. Viral loads in samples collected between 14 and 18 days after the onset of symptoms were divided into three groups: those testing negative, those with 10(4) copies/g stool. Stools from the group with 10(4) copies/g stool showed prolonged norovirus excretion (up to 32 days) in stools. Although the period of infectivity of excreted viruses has not yet been clarified, these results suggest that careful attention should be taken for at least 14 days after the onset of symptoms and that the measurement of viral load in stools around 16 days after onset might be a useful method for following the course of viral shedding for each patient infected with norovirus. (C) 2009 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved.