Hydroperoxylated vs Dihydroxylated Lipids: Differentiation of Isomeric Cardiolipin Oxidation Products by Multidimensional Separation Techniques
Hydroperoxylated vs Dihydroxylated Lipids: Differentiation of Isomeric Cardiolipin Oxidation Products by Multidimensional Separation Techniques
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DOI：
10.1021/acs.analchem.0c02605
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发表时间：
2020-09-01
影响因子：
7.4
通讯作者：
Hayen, Heiko
中科院分区：
文献类型：
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作者：
Helmer, Patrick O.;Behrens, Arne;Hayen, Heiko
近年来，心磷脂(CL)氧化产物被认为是线粒体功能障碍与年龄相关疾病的潜在标志物。氧化型化学发光的分析需要强大的分析技术，因为它具有高度的结构多样性。此外，低浓度的部分不稳定化合物构成了一个特殊的挑战，并伴随着异构体化合物的出现，例如过氧化氢和二羟基化产物。因此，我们提出了一种基于液相色谱和捕获离子迁移率谱(TIMS)的联用方法用于分离，串联质谱仪(MS/MS)用于结构表征。这使得对人工氧化的牛心CL提取物进行全面分析成为可能。异构体氧化产物可以通过迁移率分辨的MS/MS裂解实验来区分。我们开发的方法有助于更好地了解氧化CL的生理作用。
In recent years, cardiolipin (CL) oxidation products were recognized as potential markers for mitochondrial dysfunction in conjunction with age related diseases. The analysis of oxidized CL requires powerful analysis techniques due to high structural diversity. In addition, low concentrations of partly labile compounds pose a special challenge, supplemented by the occurrence of isomeric compounds, e.g., hydroperoxylated vs dihydroxylated products. Therefore, we present a hyphenated method based on liquid chromatography coupled to trapped ion mobility spectrometry (TIMS) for separation and tandem mass spectrometry (MS/MS) for structural characterization. This enables comprehensive analysis of an artificially oxidized CL extract of bovine heart. Isomeric oxidation products could be differentiated by mobility-resolved MS/MS fragmentation experiments. Our developed method could help to better understand the physiological role of oxidized CL.