Quercitrin and Taxifolin stimulate osteoblast differentiation in MC3T3-E1 cells and inhibit osteoclastogenesis in RAW 264.7 cells
Quercitrin and Taxifolin stimulate osteoblast differentiation in MC3T3-E1 cells and inhibit osteoclastogenesis in RAW 264.7 cells
复制标题
DOI：
10.1016/j.bcp.2013.09.009
复制
发表时间：
2013-11-15
影响因子：
5.8
通讯作者：
Maria Ramis, Joana
中科院分区：
文献类型：
--
作者：
Satue, Maria;del Mar Arriero, Maria;Maria Ramis, Joana
黄酮类化合物是天然的抗氧化剂，对骨代谢有积极的影响。本研究筛选不同的黄酮类化合物，以确定生物分子在骨再生的潜在用途。为此，我们使用MC 3 T3-E1和RAW264.7细胞来评估它们对细胞活力和细胞分化的影响。首先，分析不同剂量的白杨素、薯蓣皂苷、高良姜素、槲皮苷和花旗松素以确定诱导成骨细胞分化的最佳浓度。处理48小时后，剂量>= 100 μ M的薯蓣皂苷和高良姜素以及500 μ M的花旗松素显示对细胞的毒性作用。处理14天后，在用>= 100 μ M白杨素处理的细胞中观察到相同的效果。然而，安全剂量的槲皮苷（200和500 μ M）和花旗松素（100和200 μ M）诱导骨涎蛋白和骨钙素mRNA的表达。与对照组相比，在100 μ M紫杉叶素成骨细胞处理的样品中也确定了更高的骨钙素分泌水平。另一方面，槲皮苷和花旗松素降低成骨细胞中的Rankl基因表达，表明抑制破骨细胞形成。事实上，破骨细胞生成抑制槲皮苷和taxifolin治疗中观察到RAW 264.7细胞。基于这些发现，本研究表明，槲皮苷和紫杉叶素促进MC 3 T3-E1细胞中的成骨细胞分化，也抑制RAW264.7细胞中的破骨细胞生成，显示这些黄酮类化合物对骨代谢的积极作用。(C)2013 Elsevier Inc. All rights reserved.
Flavonoids are natural antioxidants that positively influence bone metabolism. The present study screened among different flavonoids to identify biomolecules for potential use in bone regeneration. For this purpose, we used MC3T3-E1 and RAW264.7 cells to evaluate their effect on cell viability and cell differentiation. First, different doses of chrysin, diosmetin, galangin, quercitrin and taxifolin were analyzed to determine the optimum concentration to induce osteoblast differentiation. After 48 h of treatment, doses >= 100 mu M of diosmetin and galangin and also 500 mu M taxifolin revealed a toxic effect on cells. The same effect was observed in cells treated with doses >= 100 mu M of chrysin after 14 days of treatment. However, the safe doses of quercitrin (200 and 500 mu M) and taxifolin (100 and 200 mu M) induced bone sialoprotein and osteocalcin mRNA expression. Also higher osteocalcin secreted levels were determined in 100 mu M taxifolin osteoblast treated samples when compared with the control ones. On the other hand, quercitrin and taxifolin decreased Rankl gene expression in osteoblasts, suggesting an inhibition of osteoclast formation. Indeed, osteoclastogenesis suppression by quercitrin and taxifolin treatment was observed in RAW264.7 cells. Based on these findings, the present study demonstrates that quercitrin and taxifolin promote osteoblast differentiation in MC3T3-E1 cells and also inhibit osteoclastogenesis in RAW264.7 cells, showing a positive effect of these flavonoids on bone metabolism. (C) 2013 Elsevier Inc. All rights reserved.