LOCAL BONE INJECTIONS OF LPS AND M-CSF INCREASE BONE-RESORPTION BY DIFFERENT PATHWAYS INVIVO IN RATS
LOCAL BONE INJECTIONS OF LPS AND M-CSF INCREASE BONE-RESORPTION BY DIFFERENT PATHWAYS INVIVO IN RATS
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DOI：
10.1152/ajpendo.1993.264.3.e391
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发表时间：
1993-03-01
影响因子：
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通讯作者：
DEVERNEJOUL, MC
中科院分区：
文献类型：
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作者：
ORCEL, P;FEUGA, M;DEVERNEJOUL, MC
我们研究了脂多糖（LPS），一种有效的单核细胞激活剂，和巨噬细胞集落刺激因子（M-CSF），一种影响单核细胞分化的造血生长因子，对正常雌性8周龄大鼠骨吸收的局部体内作用。在盐酸盐麻醉下，通过植入右股骨远端的细导管将LPS（2次注射，每次0.5 μ g）、M-CSF（2次注射，每次12.5、25、100或500 ng）或溶媒注射至骨髓间隙。抗酒石酸酸性磷酸酶（TRAP）染色后进行组织形态计量学。破骨细胞和TRAP阳性骨髓细胞（被认为是破骨细胞前体）的数量在次级海绵体中计数。LPS引起破骨细胞表面增加3倍，破骨细胞数量增加4.5倍，但TRAP阳性骨髓细胞数量无变化。M-CSF对TRAP阳性骨髓细胞数量和骨吸收诱导了显著的剂量依赖性双相效应（最低或最高浓度无变化，尽管两个中间剂量显著增加了吸收表面和破骨细胞数量）。我们的研究结果表明，局部在体内的LPS和M-CSF对骨吸收的影响，并建议这些物质的作用在不同阶段的破骨细胞的发展和功能。
We investigated the local in vivo action of lipopolysaccharide (LPS), a potent monocyte activator, and of macrophage colony-stimulating factor (M-CSF), a hemopoietic growth factor influencing monocyte differentiation, on bone resorption in normal female 8-wk-old rats. LPS (2 injections of 0.5 mug), M-CSF (2 injections of either 12.5, 25, 100, or 500 ng), or vehicle was injected into bone marrow space through a thin catheter implanted, under hydrochloride anesthesia, in the distal end of the right femur. Histomorphometry was performed after staining of the tartrate-resistant acid phosphatase (TRAP). The number of osteoclasts and of TRAP-positive marrow cells (considered as osteoclast precursors) were counted in the secondary spongiosa. LPS caused a 3-fold increase in osteoclast surface, a 4.5-fold increase in the number of osteoclasts, but no change in the number of TRAP-positive marrow cells. M-CSF induced a striking dose-dependent biphasic effect on the number of TRAP-positive marrow cells and on bone resorption (no change with the lowest or with the highest concentrations, although the two intermediate doses significantly increased resorption surfaces and the number of osteoclasts). Our results demonstrate a local in vivo effect of LPS and of M-CSF on bone resorption and suggest that these substances act at different stages of osteoclast development and function.