Antimicrobial peptide gene expression in tiger shrimp, Penaeus monodon in response to gram-positive bacterial probionts and white spot virus challenge
Antimicrobial peptide gene expression in tiger shrimp, Penaeus monodon in response to gram-positive bacterial probionts and white spot virus challenge
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DOI：
10.1016/j.aquaculture.2011.03.025
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发表时间：
2011-06-15
期刊：
影响因子：
4.5
通讯作者：
Philip, Rosamma
中科院分区：
文献类型：
--
作者：
Antony, Swapna P.;Singh, I. S. Bright;Philip, Rosamma
由于病毒性疾病爆发的不断增加以及化疗药物使用的规定，预防方法在水产养殖中变得越来越重要。在水产养殖中使用益生菌来预防和控制疾病已被广泛接受。但益生菌的确切作用方式仍然是个谜。抗菌肽（AMP）作为治疗工具的使用因其独特的作用方式和解决抗生素耐药性而成为迄今为止最有前途的研究途径之一。目前的工作旨在研究三种益生菌制剂的效果，即。采用半定量 RT-PCR，芽孢杆菌 MCCB101、微球菌 MCCB104 以及芽孢杆菌 MCCB101 + 微球菌 MCCB104 的组合对斑节对虾攻击前和攻击后白斑病毒 (WSV) 中 AMP 三个主要家族的表达的影响。研究表明，在 WSV 攻击前和攻击后，使用益生菌可显着上调 AMP 基因。发现单独或联合施用益生菌可以改变 AMP 基因的表达水平。就 AMP 基因表达和存活率而言，芽孢杆菌 MCCB101 被发现是所研究的三种益生菌中最好的。因此，还研究了施用益生菌芽孢杆菌后 AMP 基因的组织表达谱。研究发现鳃和肠支持 AMP 基因的最大上调。这项工作强调了益生菌作为一种优秀的免疫刺激模式的应用，并证明增强 AMP 的产生可能是益生菌的一种可能的作用模式。 (C) 2011 Elsevier B.V. 保留所有权利。
Prophylactic approach is gaining more and more importance in aquaculture, because of the ever increasing outbreak of viral diseases and the regulations on the use of chemotherapeutants. Use of probiotics has been widely accepted in aquaculture for the prevention and control of diseases. But the exact mode of action of probiotic bacteria still remains enigmatic. The use of antimicrobial peptides (AMPs) as a therapeutic tool has been among the most promising avenues investigated, to date, because of their unique mode of action and for addressing antibiotic resistance. The present work aims at studying the effect of three probiotic preparations viz. Bacillus MCCB101, Micrococcus MCCB104 and combination of Bacillus MCCB101 + Micrococcus MCCB104 on the expression of three major families of AMPs in Penaeus monodon pre- and post-challenge white spot virus (WSV), employing semi-quantitative RT-PCR. The study showed that the administration of probiotic bacteria, up-regulated the AMP genes considerably both pre- and post-challenge WSV. Probionts administered individually and in combination were found to alter the level of expression of AMP genes. Bacillus MCCB101 was found to be the best among the three probiotics studied in terms of AMP gene expression and survival rate. Hence, tissue-wise expression profile of AMP genes on administration of probiotic Bacillus was also studied. Gill and intestine were found to support maximum up-regulation of AMP genes. The work accentuates the application of probiotic bacteria as an excellent mode of immunostimulation and also proves that enhanced production of AMPs could be a possible mode of action of probiotic bacteria. (C) 2011 Elsevier B.V. All rights reserved.