”Composite” lymphoma, lymphoplasmacytoid and diffuse large B-cell lymphoma of the spleen: molecular-genetic evidence of a common clonal origin
”Composite” lymphoma, lymphoplasmacytoid and diffuse large B-cell lymphoma of the spleen: molecular-genetic evidence of a common clonal origin
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脾脏“复合”淋巴瘤、淋巴浆细胞样淋巴瘤和弥漫性大 B 细胞淋巴瘤：共同克隆起源的分子遗传学证据
作者：
E. Pescarmona;P. Pignoloni;A. Orazi;F. Lo Coco;Anna Maria Lavinia;M. Martelli;C. Baroni
摘要：我们在这里描述了第一例具有良好特征的脾脏“复合”淋巴瘤，其中两种成分是低级别和高级别b细胞非霍奇金淋巴瘤。患者是一名老年男性，脾脏肿大，多发低回声灶。行脾切除术，宏观和组织学检查显示同时存在“低级别”b细胞淋巴瘤，淋巴浆细胞样（免疫细胞瘤）和“高级别”b细胞淋巴瘤（免疫母细胞），它们在空间上分开。两个病变表达相同的免疫球蛋白轻链（lambda），但Southern blot分析显示免疫球蛋白重链（IgH）克隆重排模式不同。PCR分析和对高扩增重排产物的直接测序提供了分子遗传学证据，证明复合淋巴瘤的两个组成部分具有相同的克隆起源。由于EBV LMP-1和p53免疫组化均为阴性，因此本病例的肿瘤进展不太可能与EBV和p53有关。PCR分析和高扩增重排产物的直接测序是在无法进行Southern blot分析或不能提供结结性结果的情况下调查克隆的有用工具。
Abstract We describe here the first well-characterized case of ”composite” lymphoma of the spleen in which the two components were a low-grade and a high-grade B-cell non-Hodgkin’s lymphomas. The patient was an elderly man with prominent splenomegaly and multiple hypoechogenic lesions of the spleen. A splenectomy was performed, and the macroscopic and histological findings showed the simultaneous presence of a ”low-grade” B-cell lymphoma, lymphoplasmacytoid (immunocytoma) and a ”high-grade” B-cell lymphoma (immunoblastic), which were spatially separated. The two lesions expressed the same immunoglobulin light chain (lambda), but the Southern blot analysis showed different patterns of immunoglobulin heavy chain (IgH) clonal rearrangement. PCR analysis followed by direct sequencing of the IgH-amplified rearrangement products provided molecular-genetic evidence that the two components of the composite lymphoma had the same clonal origin. Since both EBV LMP-1 and p53 were negative by immunohistochemistry, it is unlikely that EBV and p53 were involved in the neoplastic progression in this case. PCR analysis and direct sequencing of IgH-amplified rearrangement products are useful tools to investigate clonality in cases in which Southern blot analysis cannot be performed or does not provide conclusive findings.
影响因子：
20.3
作者：
F. Coco;G. Gaidano;Diane;Louie;K. Offit;R. Chaganti;R. Dalla-Favera
通讯作者：
F. Coco;G. Gaidano;Diane;Louie;K. Offit;R. Chaganti;R. Dalla-Favera
通过聚合酶链反应检测新鲜、未固定和福尔马林固定、石蜡包埋组织中 B 细胞非霍奇金淋巴瘤和白血病的免疫球蛋白基因重排。
DOI：
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发表时间：
1993
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Inghirami,G;Szabolcs,MJ;Yee,HT;Corradini,P;Cesarman,E;Knowles,DM
通讯作者：
Knowles,DM
Epstein-Bar 病毒和非霍奇金淋巴瘤进展为 Ki-1 阳性、间变性大细胞表型。
DOI：
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发表时间：
1995
期刊：
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
影响因子：
--
作者：
DiGiuseppe,JA;Wu,TC;Zehnbauer,BA;McDowell,PR;Barletta,JM;Ambinder,RF;Mann,RB
通讯作者：
Mann,RB