Comparison of hypoxic effects induced by chemical and physical hypoxia on cardiomyocytes
Comparison of hypoxic effects induced by chemical and physical hypoxia on cardiomyocytes
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化学缺氧和物理缺氧对心肌细胞缺氧作用的比较
DOI：
10.1139/cjpp-2019-0092
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发表时间：
2019-10-01
影响因子：
2.1
通讯作者：
Yu, Zhi-Bin
中科院分区：
文献类型：
--
作者：
Zhao, Ru-Zhou;Jiang, Shuai;Yu, Zhi-Bin
亚硫酸钠(Na2S2O4)引起的化学性缺氧的程度和持续时间尚未见报道。目前尚不清楚氧浓度的降低(物理缺氧)会在多大程度上导致培养心肌细胞缺氧。在这项研究中，氧微电极被用来测量含有不同浓度的Na2S2O4的介质中O-2浓度的变化。然后观察和比较0.8、1.0、2.0 mM Na2S2O4和1%、3%、5%O-2对培养乳鼠心肌细胞的缺氧作用。结果表明，在180分钟的观察期内，Na2S2O4处理未能使O2-浓度保持恒定。仅2.0 mM Na2S2O4组显著增加低氧诱导因子1α(HIF-1α)的表达和低氧反应。值得注意的是，3%O-2只显著增加心肌细胞HIF-1α的表达，而1%O-2不仅增加心肌细胞HIF-1α的表达，而且还增加心肌细胞的凋亡率。这些结果表明，Na2S2O4不适合建立培养的新生大鼠心肌细胞的低氧模型，低氧或低氧条件下培养的新生大鼠心肌细胞具有明显的低氧效应，可作为建立低氧细胞模型的起始氧浓度。
The degree and duration of chemical hypoxia induced by sodium dithionite (Na2S2O4) have not been reported. It is not yet clear how much reduction in the O-2 concentration (physical hypoxia) can lead to hypoxia in cultured cardiomyocytes. In this study, oxygen microelectrodes were used to measure changes in the O-2 concentration in media containing different concentrations of Na2S2O4. Then, hypoxic effects of 0.8, 1.0, and 2.0 mM Na2S2O4 or 1%, 3%, and 5% O-2 in cultured cardiomyocytes from neonatal rats were observed and compared. The results showed that the O-2 concentration failed to remain constant by Na2S2O4 treatment during the 180-minute observation period. Only the 2.0 mM Na2S2O4 group significantly increased the expression of hypoxia-inducible factor 1 alpha (HIF-1 alpha) and hypoxic responses. Notably, 3% O-2 only significantly increased the expression of HIF-1 alpha in cardiomyocytes, while 1% O-2 not only increased the expression of HIF-1 alpha but also increased the apoptotic rate in cardiomyocytes. These results suggest that Na2S2O4 is not suitable for establishing a hypoxic model in cultured neonatal rat cardiomyocytes, and neonatal rat cardiomyocytes cultured at or below 1% O-2 induced significant hypoxic effects, which can be used as a starting O-2 concentration for establishing a hypoxic cell model.