Chiral separation of atropine by high-performance liquid chromatography
Chiral separation of atropine by high-performance liquid chromatography
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DOI：
10.1016/j.chroma.2005.02.073
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发表时间：
2005-09-23
影响因子：
4.1
通讯作者：
Lafosse, A
中科院分区：
文献类型：
--
作者：
Breton, D;Buret, D;Lafosse, A
对映体的分离和定量以及对映体纯度的测定是当前药物分析中不可缺少的任务。在各种技术中，液相色谱法由于若干优点仍然是最好的方法。高速度、高灵敏度和重复性结果使LC成为几乎所有实验室的首选方法。含有1酸性糖蛋白作为手性选择剂的相适合分离结构差异很大的带电和不带电对映体。阿托品被广泛用作副交感神经解药、抗胆碱能药和止吐药。它是立即处理与神经毒剂有关的毒性的首选解毒剂之一。立体选择分离是通过预先包装的α(1)-酸糖蛋白柱实现的，无需任何衍生化程序。液相色谱系统耦合质谱与常压化学电离界面在正离子模式。采用析因实验设计优化后，在选择性离子监测中检测色谱分析物。少量对映体组成可用质谱法或紫外光谱法测定（小于5%）。(c) 2005 Elsevier B.V.版权所有
The separation and quantitation of the enantiomers and also the determination of the enantiomeric purity are now current and indispensable tasks for the pharmaceutical analysis. Among the various techniques, liquid chromatography remains the best modality owing to several advantages. High speed, sensitivity, and reproducible results make LC the method of choice in almost all laboratories. Phases that contain a I acid glycoprotein as chiral selector are suitable for separation of charged and uncharged enantiomers with widely different structure. Atropine is widely used as parasympatolytic, anticholinergic and antiemetic drugs. It is one of the preferred antidote for immediate management of toxicity associated with nerve agents. Stereoselective separation was achieved with a prepacked alpha(1)-acid glycoprotein column without any derivatization procedure. The liquid chromatography system is coupled to mass spectrometry with an atmospheric pressure chemical ionization interface in the positive-ion mode. The chromatographed analytes are detected in selective ion monitoring after optimisation using factorial experimental design. Small amount of enantiomeric composition can be evaluated either by MS or by UV spectrometry (less than 5%). (c) 2005 Elsevier B.V. All rights reserved.