Genetic variation and relationships among cultivated, wild, and semiwild soybean
Genetic variation and relationships among cultivated, wild, and semiwild soybean
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DOI：
10.2135/cropsci2004.0316
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发表时间：
2004-01-01
期刊：
影响因子：
2.3
通讯作者：
Nelson, RL
中科院分区：
文献类型：
--
作者：
Chen, YW;Nelson, RL
一些一年生大豆种质介于大豆（Glycine max（L.）Merr.和大豆（Glycine soja Sieb.）& Zucc.被贴上了半野生的标签很少有研究同时探讨了G. soja、大豆G. max和半野生型。本研究的目的是量化G. soja、大豆G. max和半野生种质资源，探讨G. soja、大豆G. max和半野生种质;并根据形态性状和DNA标记研究了表型间的关系。92个半野生的，G。soja和G.用137个RAPD标记对美国农业部大豆种质资源中心的120份大豆种质资源的20个表型性状进行了评价。分别计算表型性状和DNA数据的马氏距离和Jaccard遗传相似性矩阵。采用非层次聚类和层次聚类以及多维标度（MDS）分析了半野生、G. soja和G.最大加入。主成分分析，以确定最显着的形态特征，在分离三组。对于加入检查，独特的RAPD标记被发现为每个分类类型。由表型或DNA数据定义的三个聚类高度一致，并与G. soja、大豆G. max和semiwild分类。在RAPD数据分析的基础上，G.大豆种质资源的遗传多样性最高，半野生种质资源的遗传多样性最低。半野生型和野生型大豆品种间的亲缘关系较近。大豆种质这些数据将有助于确定年度甘氨酸的核心收集。
Some annual Glycine accessions are intermediate between the standard phenotypes of Glycine max (L.) Merr. and Glycine soja Sieb. & Zucc. and have been labeled semiwild. Few studies have examined both the genetic and phenotypic relationships among G. soja, G. max, and semiwild-types by combining morphological traits and DNA markers. The objectives of this research were to quantify genetic variation within G. soja, G. max, and semiwild accessions; to investigate the relationships among the G. soja, G. max, and semiwild accessions; and to examine the relationships among phenotypes on the basis of morphological traits and genotypes on the basis of DNA markers. Ninety-two semiwild, G. soja, and G. max accessions from the USDA Soybean Germplasm Collection were evaluated for 20 phenotypic traits and with 137 RAPD markers. Mahalanobis distances and a Jaccard genetic similarity matrix were calculated for phenotypic traits and DNA data, respectively. Nonhierarchical and hierarchical clustering as well as multidimensional scaling (MDS) were used to evaluate relationships among semiwild, G. soja, and G. max accessions. Principal component analysis was applied to identify the morphological traits that were most significant in separating the three groups. For the accessions examined, unique RAPD markers were found for each taxonomic type. Three clusters defined by either phenotypic or DNA data are highly consistent and strongly corresponded to G. soja, G. max, and semiwild classifications. On the basis of the analysis of RAPD data, G. soja accessions have the greatest genetic diversity and semiwild accessions the least. Glycine mar and semiwild accessions are more closely related to each other than to G. soja accessions. These data will be useful in helping to define a core collection of annual Glycine.