Up-regulation of pro-angiogenic pathways and induction of neovascularization by an acute retinal light damage
Up-regulation of pro-angiogenic pathways and induction of neovascularization by an acute retinal light damage
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DOI：
10.1038/s41598-020-63449-y
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发表时间：
2020-04-14
影响因子：
4.6
通讯作者：
Maccarone, R.
中科院分区：
文献类型：
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作者：
Tisi, A.;Parete, G.;Maccarone, R.
光损伤（LD）模型主要用于研究年龄相关性黄斑变性（AMD）的一些主要方面，如氧化应激和感光细胞死亡。存在几种光诱导的视网膜变性的方案。急性光损伤的特征是短暂暴露（24小时）于高强度光（1000勒克斯），并导致视网膜的局灶性变性，随着时间的推移而进展。到目前为止，还没有实验数据将该模型与新生血管事件联系起来。因此，本研究的目的是表征急性光损伤后的视网膜，以评估血管化是否受到影响。进行了功能、分子和形态学研究。在所有恢复时间（LD后7、60、120天）评估视网膜电图反应。从光损伤后7天开始，功能反应显著降低，直到恢复120天仍保持较低水平。光暴露后7天，新生血管侵入感光层，视网膜新生血管发生。值得注意的是，随着退变的进展，新血管生成与VEGF、bFGF及其各自的受体（VEGFR2和FGFR1）的上调相关。这些重要的结果表明，短暂暴露于明亮的光诱导上调促血管生成途径与随后的新血管形成。
The light damage (LD) model was mainly used to study some of the main aspects of age related macular degeneration (AMD), such as oxidative stress and photoreceptor death. Several protocols of light-induced retinal degeneration exist. Acute light damage is characterized by a brief exposure (24hours) to high intensity light (1000 lux) and leads to focal degeneration of the retina which progresses over time. To date there are not experimental data that relate this model to neovascular events. Therefore, the purpose of this study was to characterize the retina after an acute light damage to assess whether the vascularization was affected. Functional, molecular and morphological investigations were carried out. The electroretinographic response was assessed at all recovery times (7, 60, 120 days after LD). Starting from 7 days after light damage there was a significant decrease in the functional response, which remained low up to 120 days of recovery. At 7 days after light exposure, neo-vessels invaded the photoreceptor layer and retinal neovascularization occurred. Remarkably, neoangiogenesis was associated to the up-regulation of VEGF, bFGF and their respective receptors (VEGFR2 and FGFR1) with the progression of degeneration. These important results indicate that a brief exposure to bright light induces the up-regulation of pro-angiogenic pathways with subsequent neovascularization.