In vitro maintenance of terminal-differentiated state in hepatocytes entrapped within calcium alginate.
In vitro maintenance of terminal-differentiated state in hepatocytes entrapped within calcium alginate.
复制标题
海藻酸钙包埋的肝细胞的终末分化状态的体外维持。
DOI：
10.1111/j.1525-1594.1987.tb00946.x
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发表时间：
2008
影响因子：
2.4
通讯作者：
K. Yagi
中科院分区：
文献类型：
--
作者：
Y. Miura;T. Akimoto;Y. Fuke;S. Yamazaki;K. Yagi
在海藻酸钙包裹的培养肝细胞中，通过增加细胞密度来刺激肝脏特异的功能，如酪氨酸转氨酶和丝氨酸脱水酶的诱导。相反，降低细胞密度会强烈刺激与生长相关的功能，如葡萄糖-6-磷酸脱氢酶的诱导。将从成年大鼠肝脏提纯的质膜添加到低细胞密度的包埋培养中，可以模拟这种相互调节。此外，添加肾上腺素(α，β激动剂)和心得安(β受体阻滞剂)可刺激乳酸的糖异生。这些结果表明，包埋的肝细胞不仅维持终末分化状态，而且如体内所示，维持α-肾上腺素能反应。
In cultured hepatocytes entrapped within Ca-alginate, liver-specific functions such as induction of tyrosine aminotransferase and serine dehydratase were stimulated by increasing the cell density. In contrast, a growth-related function such as induction of glucose-6-phosphate dehydrogenase was strongly stimulated by decreasing the cell density. This reciprocal regulation was mimicked by the addition of plasma membranes purified from adult rat liver to entrapment cultures at low cell density. Also, gluconeogenesis from lactate was stimulated by the addition of epinephrine (alpha,beta-agonist) with propranolol (beta-blocker). These results suggest that entrapped hepatocytes maintain not only terminal-differentiated state but also alpha-adrenergic response as shown in vivo.