Wheat Flour, Enriched with γ-Oryzanol, Phytosterol, and Ferulic Acid, Alleviates Lipid and Glucose Metabolism in High-Fat-Fructose-Fed Rats
Wheat Flour, Enriched with γ-Oryzanol, Phytosterol, and Ferulic Acid, Alleviates Lipid and Glucose Metabolism in High-Fat-Fructose-Fed Rats
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富含γ-谷维素、植物甾醇和阿魏酸的小麦粉可减轻高脂果糖喂养大鼠的脂质和葡萄糖代谢
DOI：
10.3390/nu11071697
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发表时间：
2019-07-01
期刊：
影响因子：
5.9
通讯作者：
Zhou, Feng
中科院分区：
文献类型：
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作者：
Guo, Xiao-Xuan;Zeng, Zhu;Zhou, Feng
(1)背景资料：高脂肪、果糖和精制碳水化合物摄入的现代饮食模式与脂质/葡萄糖代谢紊乱密切相关。本研究的主要目的是为设计具有一定脂糖代谢调节作用的肥胖人群功能食品提供新的思路。(2)研究方法：采用高脂果糖饮食诱导的雄性SD大鼠模型，观察谷维醇、植物甾醇和阿魏酸强化小麦粉对大鼠糖脂代谢紊乱的缓解作用。用蛋白质印迹法阐明了潜在的机制。(3)结果如下：在体外细胞模型中，γ-谷维素、植物甾醇和阿魏酸通过增加AMPK和Akt的磷酸化、PI 3 K表达以及降低DGAT 1和SCD的表达来调节脂质/葡萄糖代谢。体内研究表明，富含阿魏酸和γ-谷维素的面粉有利于管理体重，改善糖代谢、高脂血症和肝脏脂质蓄积。植物甾醇强化面粉对高胰岛素血症、胰岛素抵抗和高尿酸血症有明显的调节作用。来自肝脏样品的蛋白质的蛋白质印迹分析揭示，这些富集的面粉通过提高AMPK和Akt的磷酸化来减轻肝脏脂质积累和胰岛素抵抗。(4)结论：本研究表明，这些强化面粉可以作为一种促进健康的功能食品，调节肥胖相关的脂/糖代谢功能障碍的大鼠。
(1) Background: Modern dietary patterns with a high intake of fat and fructose, as well as refined carbohydrates, closely relate to lipid/glucose metabolic disorders. The main objective of this study is to provide new thoughts in designing functional food with some lipid/glucose metabolism regulating effects for obese people. (2) Methods: The alleviating abilities of γ-oryzanol, phytosterol or ferulic acid-enriched wheat flour on lipid/glucose metabolic dysfunction were evaluated in male SD rats induced by a high-fat-fructose diet. The underlying mechanisms were clarified using western blot. (3) Results: In an in vitro cell model, γ-oryzanol, phytosterol and ferulic acid regulate lipid/glucose metabolism by increasing the phosphorylation of AMPK and Akt, and PI3K expression, as well as decreasing expressions of DGAT1 and SCD. The in vivo study shows that ferulic acid and γ-oryzanol-enriched flours are beneficial for managing body weight, improving glucose metabolism, hyperlipidemia and hepatic lipid accumulation. Phytosterol-enriched flour exerted remarkable effects in regulating hyperinsulinemia, insulin resistance and hyperuricemia. Western blot analysis of proteins from liver samples reveals that these enriched flours alleviated hepatic lipid accumulation and insulin resistance through their elevation in the phosphorylation of AMPK and Akt. (4) Conclusions: Our study indicates that these enriched flours can serve as a health-promoting functional food to regulate obesity-related lipid/glucose metabolic dysfunction in rats.