Oxidative stress and hematologic and biochemical parameters in individuals with Down syndrome
Oxidative stress and hematologic and biochemical parameters in individuals with Down syndrome
复制标题
DOI：
10.4065/80.12.1607
复制
发表时间：
2005-12-01
影响因子：
8.9
通讯作者：
Salvador, M
中科院分区：
文献类型：
--
作者：
Garcez, ME;Peres, W;Salvador, M
目的：评估唐氏综合症患者的硫代巴比妥酸反应物质（TBARS）、尿酸、丝氨酸超氧化物歧化酶和过氧化氢酶活性水平，以及血清总铁、总铁结合力（TIBC）、红细胞渗透脆性和血象。 参与者和方法：该研究采样了（2003年7月至12月）50名唐氏综合症患者患有唐氏综合症的人以及 50 名年龄和性别相匹配的无唐氏综合症的人（对照组）。 TBARS的水平通过TBARS方法测量。通过分光光度法测定血清超氧化物歧化酶和过氧化氢酶活性以及尿酸水平。通过溶血百分比评估红细胞渗透脆性。用自动化系统测定血象、血清总铁水平和TIBC。结果：结果显示，与对照组相比，唐氏综合症组的TBARS、尿酸、血清超氧化物歧化酶和过氧化氢酶活性水平较高。我们还观察到唐氏综合症患者的红细胞渗透脆性略有增加，但两组的血清总铁水平、TIBC 和血象均在与年龄相关的参考值之内。结论：据我们所知，这是首次在唐氏综合症患者中观察到血清超氧化物歧化酶和过氧化氢酶活性的增加。尽管其他研究是必要的，但我们的结果增加了对唐氏综合症患者氧化应激增加的机制的理解，并且可能有助于支持未来改善唐氏综合症患者生活的抗氧化疗法。
OBJECTIVE: To evaluate the levels of thiobarbituric acid reactive substances (TBARS), uric acid, and seric superoxide dismutase and catalase activities, as well as total serum Iron, total Iron-binding capacity (TIBC), erythrocyte osmotic fragility, and hemograms In people with Down syndrome.PARTICIPANTS AND METHODS: The study sampled (July to December 2003) 50 people with Down syndrome and 50 Individuals without Down syndrome (control group) matched by age and sex. The levels of TBARS were measured by the TBARS method. Serum superoxide dismutase and catalase activities and uric acid levels were determined spectrophotometrically. Erythrocyte osmotic fragility was assessed by the percentage of hemolysis. Hemograms, total serum Iron level, and TIBC were determined with automated systems.RESULTS: The results showed that levels of TBARS, uric acid, and seric superoxide dismutase and catalase activities were higher in the Down syndrome group compared with the control group. We also observed a slight Increase in erythrocyte osmotic fragility in the Down syndrome group, but the total serum Iron levels, TIBC, and hemograms for both groups were within the age-related reference values.CONCLUSION: This was the first time, to our knowledge, that increases in seric superoxide dismutase and catalase activities were observed in people with Down syndrome. Although other studies are necessary, our results add to the understanding of the mechanisms responsible for the Increased oxidative stress observed in individuals with Down syndrome and may be useful in supporting future antioxidant therapies that will Improve the lives of people with Down syndrome.