EFFECT OF ACUTE INFLAMMATORY LUNG INJURY ON THE EXPRESSION OF MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1) IN RAT PULMONARY ALVEOLAR MACROPHAGES
EFFECT OF ACUTE INFLAMMATORY LUNG INJURY ON THE EXPRESSION OF MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1) IN RAT PULMONARY ALVEOLAR MACROPHAGES
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DOI：
10.1165/ajrcmb/7.2.134
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发表时间：
1992-08-01
影响因子：
6.4
通讯作者：
FANTONE, JC
中科院分区：
文献类型：
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作者：
BRIELAND, JK;JONES, ML;FANTONE, JC
使用一个充分表征的免疫复合物介导的急性炎性肺损伤大鼠模型，我们确定支气管肺泡灌洗液中单核细胞趋化活性具有时间依赖性。单核细胞趋化活性也显着增强培养上清液从肺泡巨噬细胞（PAM）从受伤的大鼠肺。北方杂交分析显示，大鼠单核细胞趋化蛋白1（MCP-1）mRNA的表达显着增加，从大鼠免疫复合物诱导的肺损伤的PAM。肺损伤后PAMs培养上清液中MCP-1 mRNA表达增加，单核细胞趋化活性增加，提示PAMs可能通过分泌MCP-1等单核细胞趋化因子参与急性炎性肺损伤的发病机制。
Using a well-characterized rat model of immune complex-mediated acute inflammatory lung injury, we determined that there is a time-dependent elaboration of monocyte chemotactic activity in bronchoalveolar lavage fluid. Monocyte chemotactic activity is also significantly enhanced in culture supernatants from pulmonary alveolar macrophages (PAMs) from injured rat lungs. Northern hybridization analysis revealed markedly increased expression of rat monocyte chemoattractant protein 1 (MCP-1) mRNA in PAMs obtained from rats with immune complex-induced lung injury. The increased expression of MCP-1 mRNA and associated increase in monocyte chemotactic activity present in culture supernatants of PAMs from injured rat lungs suggest that PAMs may participate in the pathogenesis of acute inflammatory lung injury by the secretion of monocyte chemoattractants including MCP-1.